摘要
为研究提取溶剂对栀子壳果胶理化性质的影响,采用柠檬酸、氢氧化钠和水分别提取栀子壳果胶,测定不同提取剂的果胶提取量、单糖组成和相对分子量,用傅里叶红外(FTIR)、扫描电镜和X-衍射比较结构特征,并分析了抗氧化活性功能。结果表明:柠檬酸、水和氢氧化钠提取对栀子壳果胶的得率分别为17.20%、16.93%、16.67%,3种栀子壳果胶均由8种单糖组成,相对分子量从大到小依次为水提果胶、碱提果胶、酸提果胶;3种栀子壳果胶官能团一致;电镜扫描发现栀子壳果胶表面粗糙并伴有微卷结构和颗粒,其中碱提果胶表面最光滑。X射线衍射分析发现3种栀子壳果胶均具有半结晶及无定形结构。柠檬酸提取的果胶DPPH清除能力最强,碱提取的果胶最弱。
In order to study the effect of extraction solution on the physicochemical properties of gardenia shell pectin,gardenia shell pectin was extracted with citric acid,sodium hydroxide and water,respectively.The extraction yield,monosaccharide composition and molecular weight of pectin extracted by using different solutions were determined.The structural characteristics were compared by using Fourier transform infrared(FTIR),scanning electron microscopy and X-ray diffraction,and the antioxidant activity function was analyzed.The results showed that the yield of pectin from gardenia shell extracted by citric acid,water and sodium hydroxide was 17.20%,16.93%and 16.67%,respectively.All of pectins were composed of 8 monosaccharides,and the molecular weight of pectin was water extracted pectin>alkali extracted pectin>acid extracted pectin;Three gardenia shell pectins showed a similar functional groups.According to the scanning electron microscope,the surface of gardenia shell pectin is rough and accompanied by micro-roll structure and particles,of which the alkali extracted has the smoothest surface.The X-ray diffraction results showed that all of pectins had semi-crystalline and amorphous structure.Pectin extracted by citric acid has the strongest DPPH scavenging ability,and that extracted by alkali is the weakest.
作者
潘康
徐裕茹
沈伟康
章智
许光治
PAN Kang;XU Yuru;SHEN Weikang;ZHANG Zhi;XU Guangzhi(College of Food and Health,Zhejiang A&F University,Hangzhou 311300,Zhejiang)
出处
《浙江农业科学》
2024年第6期1482-1487,共6页
Journal of Zhejiang Agricultural Sciences
关键词
栀子壳
果胶
理化特性
gardenia shell
pectin
physicochemical properties
