摘要
【目的】从黄河三角洲盐碱地中筛选到具有抗盐促生作用的一株根际菌,制成菌剂用于缓解玉米植株的高盐胁迫。【方法】通过功能培养基、16S rDNA序列和全基因组分析,确定菌株种属以及对玉米植株的抗盐促生效果。【结果】从黄河三角洲盐碱地中筛选到一株可在0-16%的NaCl和pH 5-8的条件下正常生长的萎缩芽孢杆菌CNY01,该菌株可促进玉米在高盐条件下生长,具有降蛋白和解钾的能力,还可抑制青枯雷尔氏菌、串珠镰刀菌等病原菌的生长。玉米植株在含100 mmol/L NaCl的营养液中生长8 d时,施加菌株CNY01可使植株的生理株高、根长和鲜重分别提高38.80%(P<0.01)、23.73%和28.19%(P<0.01)。玉米植株在加100 mmol/L NaCl的高盐盆栽条件下生长28 d时,菌株CNY01可使植株的株高、地上鲜重和地下鲜重分别提高10.84%、41.87%(P<0.01)和23.29%。全基因组序列分析也预测出该菌株基因组中含有维持细胞渗透压、合成应激蛋白等缓解高盐胁迫的基因。【结论】从黄河三角洲盐碱地中筛选到一株具有防病促生功能的萎缩芽孢杆菌CNY01,对玉米植株有显著的抗盐促生作用,结合基因组结果预测到该菌株含有与抗盐促生相关基因,是重要的菌种资源。
【Objective】A rhizobacterium with salt-resistant and growth-promoting effects was screened from the saline-alkali soil in Yellow River Delta to prepare an agent for alleviating high-salt stress of maize seedlings.【Method】The salt-resistant and growth-promoting functions of the strain were identified on functional mediums,and the species of the strain was determined by 16S rDNA sequence and whole genome analysis.The strain was applied to maize rhizosphere to verify the salt-resistant and growth-promoting effects of the strain on maize seedlings.【Result】A strain Bacillus atrophaeus CNY01 was screened from the saline-alkali soil of Yellow River Delta.Strain CNY01 grew normally under the condition of 0-16%NaCl and pH 5-8.Strain CNY01 had the ability of degrading protein and dissolving potassium,and inhibited the growth of plant pathogens such as Ralstonia solanacearum and Fusarium moniliforme.The application of strain CNY01 promoted the growth of maize seedlings under high-salt condition and alleviated the stress of high salt on maize seedlings.When strain CNY01 was applied to maize seedlings in nutrient solution containing 100 mmol/L NaCl for 8 d,the physiological plant height,root length,and fresh weight of maize seedlings increased by 38.80%(P<0.01),23.73%,and 28.19%(P<0.01),respectively.When strain CNY01 was applied to maize seedlings in pot experiments adding 100 mmol/L NaCl for 28 d,the physiological plant height,the aboveground fresh weight,and the underground fresh weight of maize seedlings increased by 10.84%,41.87%(P<0.01),and 23.29%,respectively.Genes that maintain cell osmotic pressure and synthesize stress proteins to alleviate high salt stress were also predicted in the genome sequence of this strain.【Conclusion】A strain of B.atrophaeus CNY01 is screened from saline-alkali soil of Yellow River Delta,which has the function of preventing disease and promoting growth,and has significant salt-resistant and promoting growth effect on maize plants.Meanwhile,strain CNY01 contains genes related to salt-resistant and growth-promoting.B.atrophaeus CNY01 is an important strain resource.
作者
孙亚楠
王春雪
王欣
杜秉海
刘凯
汪城墙
SUN Ya-nan;WANG Chun-xue;WANG Xin;DU Bing-hai;LIU Kai;WANG Cheng-qiang(College of Life Sciences,National Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources,Shandong Engineering Research Center of Plant-Microbia Restoration for Saline-alkali Land,Shandong Provincial Key Laboratory of Agricultural Microbiology,Shandong Agricultural University,Tai'an 271018)
出处
《生物技术通报》
CAS
CSCD
北大核心
2024年第5期248-260,共13页
Biotechnology Bulletin
基金
山东省重点研发计划(2023TZXD003)
国家自然科学基金项目(32170133,31700094)。
关键词
玉米
植物根际促生细菌
萎缩芽孢杆菌
抗盐促生
全基因组测序
maize
plant growth-promoting rhizobacteria
Bacillus atrophaeus
salt-resistant and growth-promoting
whole-genome sequencing
