摘要
为更好地利用无花果副产物,提高其经济价值,探究了无花果多糖(FCW)抗氧化性能。采用微波辅助提取FCW,并对提取工艺进行优化。最佳条件为提取温度70℃、料液比1∶40、微波功率800 W和提取时间50 min,在此条件下,FCW提取率12.32%。以残渣模拟无花果生产后的废料,采用碱液浸提法提取无花果残渣多糖(FCA),并分析其热稳定性。结果表明,FCA在260℃以下具有良好的热稳定性。采用离子交换柱和凝胶柱对FCA进行分离纯化得到分子量28.188 kDa的多糖组分PFCA2,单糖组成结果表明,PFCA2主要由鼠李糖、葡萄糖、阿拉伯糖、半乳糖、葡萄糖醛酸和半乳糖醛酸组成。体外抗氧化试验表明,PFCA2具有良好的DPPH自由基和羟基自由基清除活性,其IC50值分别为0.5041和3.2802 mg/mL;FCA具有良好的抗氧化能力,是天然的抗氧化剂。该研究结果为无花果工业副产物精深加工提供理论依据。
To better utilize by-products of Ficus carica L.and improve their economic value,antioxidant properties of Ficus carica L.polysaccharides(FCW)were investigated.Microwave-assisted extraction of FCW was carried out,and extraction process was optimized.The optimal conditions were that,extraction temperature was 70℃,solid-liquid ratio was 1∶40,microwave power was 800 W,and extraction time was 50 min.Under these conditions,extraction rate of FCW was 12.32%.The residue was used to simulate waste after Ficus carica L.production,Ficus carica L.residue polysaccharides(FCA)was extracted using alkaline extraction method,and analyze their thermal stability.Results showed that FCA had good thermal stability below 260℃.Polysaccharide component PFCA2 with a molecular weight of 28.188 kDa was obtained by separating and purifying it by ion exchange column and gel column.Results of monosaccharide composition showed that PFCA2 was mainly composed of rhammnose,glucose,arabinose,galactose,glucuronic acid,and galacturonic acid.In vitro antioxidant experiments showed that PFCA2 had good scavenging activities for DPPH free radicals and hydroxyl free radicals,with IC50 values of 0.5041 mg/mL and 3.2802 mg/mL.This study showed that FCA have good antioxidant capacity and were natural antioxidants.This study could provide a theoretical basis for deep processing of industrial by-products of Ficus carica L..
作者
沈静
郭建行
冀晓龙
SHEN Jing;GUO Jianhang;JI Xiaolong(School of Bioengineering,Yangling Vocational&Technical College,Yangling Shaanxi 712100,China;College of Food and Bioengineering,Zhengzhou University of Light Industry,Zhengzhou Henan 450001,China)
出处
《农业工程》
2024年第5期100-107,共8页
AGRICULTURAL ENGINEERING
基金
杨凌职业技术学院2022年院内基金项目(ZK22-49)。
关键词
无花果
多糖
分离纯化
理化性质
抗氧化活性
Ficus carica L.
polysaccharide
isolation and purification
physical and chemical properties
antioxidant activity
