摘要
以息半夏的块茎为原料,乙醇为超声波辅助提取溶剂,考察乙醇体积分数、超声时间、料液比、超声温度等单因素对总生物碱提取率的影响,设计正交试验优化提取工艺,并测试总生物碱的体外抗氧化和降血糖活性。结果表明,最佳提取条件为乙醇体积分数65%、提取时间60 min、料液比1∶45、超声温度65℃,总生物碱的提取率达0.502%;总生物碱对DPPH、ABTS自由基的清除率分别达89.90%和43.60%,对α-葡萄糖苷酶的抑制率达86.40%。该工艺简单可行,总生物碱表现出良好的体外抗氧化和降血糖生物活性。
Taking the tuber of Pinellia ternata as raw material and ethanol as ultrasonic-assisted extraction solvent,the influence of single factors such as ethanol volume fraction,ultrasonic time,material-liquid ratio and ultrasonic temperature on total alkaloids ex-traction rate was investigated.The orthogonal test was designed to optimize the extraction process,and the in vitro antioxidant and hy-poglycemic activities of total alkaloids were tested.The results showed that the optimal extraction condition was ethanol volume fraction of 65%,extraction time of 60 min,the material-liquid ratio of 1∶45,ultrasonic temperature of 65℃,and the extraction rate of total alkaloids was 0.502%;the clearance of DPPH and ABTS radicals reached 89.90%and 43.60%,respectively,and the inhibition ofα-glucosidase reached 86.40%.The process was simple and feasible,and the total alkaloids showed good in vitro antioxidant and hypo-glycemic biological activities.
作者
赵丽平
张雯雯
陈琼
孙伟
张文谦
马晓雯
ZHAO Li-ping;ZHANG Wen-wen;CHEN Qiong;SUN Wei;ZHANG Wen-qian;MA Xiao-wen(School of Pharmaceutical Engineering,Xinyang Agriculture and Forestry University,Xinyang 464000,Henan,China)
出处
《湖北农业科学》
2024年第5期151-155,161,共6页
Hubei Agricultural Sciences
基金
河南省科技攻关项目(212102310343)
河南省创新示范专项(201111310900)
校级高水平科研孵化器建设基金资助项目(FCL202008)
校级优秀基层组织建设项目(YXJCJXZZ202003)。
关键词
息半夏
总生物碱
正交试验
抗氧化
降血糖
Pinellia ternata
total alkaloids
orthogonal test
antioxidant
hypoglycemic action
