摘要
为建立同时检测陕西平利绞股蓝(Gynostemma pentaphyllum)中13种酚酸含量的测定方法,采用高效液相色谱法(HPLC),利用甲醇水溶液(80%)常温涡旋,超声波提取,色谱柱为Dimensions C18(4.6 mm×150 mm,5μm,P/N:227-30011-07,C/N:18C03033),流动相为甲醇(100%)和磷酸水溶液(0.5%),洗脱方法为梯度洗脱,检测波长为280 nm,柱温为30℃,流速为1.0 mL/min,进样体积为5μL。结果表明,13种酚酸在浓度为5~100 mg/L时线性关系良好,相关系数(R2)≥0.998;在样本加标回收试验中,平均加标回收率为61.8%~131.0%。该测定方法精密度、重复性、稳定性好,适用于陕西平利绞股蓝中酚酸含量的测定。
In order to establish a method for simultaneously detecting the content of 13 phenolic acids in Gynostemma pentaphyllum from Pingli,Shaanxi,high performance liquid chromatography(HPLC)was used.Methanol aqueous solution(80%)was used for room temperature vortex and ultrasonic extraction,the chromatographic column was Dimensions C18(4.6 mm×150 mm,5μm,P/N:227-30011-07,C/N:18C03033),with a mobile phase of methanol(100%)and phosphoric acid aqueous solution(0.5%).The elu-tion method was gradient elution,with a detection wavelength of 280 nm,column temperature of 30℃,flow rate of 1.0 mL/min,and injection volume of 5μL.The results showed that there was a good linear relationship among the 13 phenolic acids at concentrations of 5~100 mg/L,with a correlation coefficient(R2)≥0.998;in the sample spiking recovery experiment,the average spiking recovery rate was 61.8%~131.0%.This determination method had good precision,repeatability,and stability,and was suitable for the determi-nation of phenolic acids content in Gynostemma pentaphyllum from Pingli,Shaanxi.
作者
王珂
周琼
李建科
WANG Ke;ZHOU Qiong;LI Jian-ke(School of Modern Agriculture and Biotechnology,Ankang University,Ankang 725000,Shaanxi,China)
出处
《湖北农业科学》
2024年第5期182-186,共5页
Hubei Agricultural Sciences
基金
陕西省科技厅创新人才推进计划基金项目(2019TD-042
2023AYPT06)。
