摘要
以植物乳杆菌CICC 20022为研究对象,探讨在胆盐胁迫环境中添加杜仲叶多糖对菌株活菌数、微观结构、细胞膜脂肪酸组成、细胞膜和细胞壁结构完整性的影响。结果表明:在胆盐胁迫环境中添加杜仲叶粗多糖或杜仲叶精多糖并培养24 h时,植物乳杆菌CICC 20022的活菌数显著增加(P<0.05),分别是对照组的1.30倍或1.10倍;与对照组相比,添加杜仲叶粗多糖或杜仲叶精多糖均可较好地保护菌体的微观结构,显著减少因胆盐胁迫造成的菌体损伤,且菌体的PI荧光强度、碱性磷酸酶(AKP)活性和DNA渗漏量均显著降低(P<0.05),细胞膜中不饱和脂肪酸/饱和脂肪酸的比例(U/S)显著增加(P<0.05),达0.37。因此,在胆盐胁迫环境中添加杜仲叶多糖能较好地保持植物乳杆菌CICC 20022细胞结构的完整性,并提高其胆盐耐受性。
With Lactobacillus plantarum CICC 20022 was used as the research object,this study investigated the effects of Eucommia ulmoides leaf polysaccharides on the number of viable bacteria,microstructure,fatty acid composition of cell membrane,and structural integrity of cell membrane and cell wall under bile salt stress.The results showed that the viable count of L.plantarum CICC 20022 was significantly increased(P<0.05),when the crude polysaccharide or the refined polysaccharide of E.ulmoides leaf were added to the bile salt stress environment and cultured for 24 h,which was 1.30 times or 1.10 times that of the control,respectively.Compared with the control,the addition of crude polysaccharide from E.ulmoides leaf or refined polysaccharide from E.ulmoides leaf could significantly protect the microstructure of bacteria and reduce the cell damage caused by bile salt stress,and the PI fluorescence intensity,alkaline phosphatase(AKP)activity and DNA leakage of cells were significantly reduced(P<0.05),while the ratio of unsaturated fatty acids to saturated fatty acids(U/S)in cell membrane was significantly increased(P<0.05)to 0.37.The results indicated that adding crude polysaccharide from E.ulmoides leaf in the environment of bile salt stress could maintain the integrity of cell structure of L.plantarum CICC 20022 and improve its bile salt tolerance.
作者
张丽华
刘世豪
唐培鑫
李昌文
赵光远
纵伟
ZHANG Lihua;LIU Shihao;TANG Peixin;LI Changwen;ZHAO Guangyuan;ZONG Wei(College of Food and Bioengineering,Zhengzhou University of Light Industry,Zhengzhou 450001,China;Henan Key Laboratory of Cold Chain Food Quality and Safety Control,Ministry of Education,Zhengzhou 450001,China)
出处
《轻工学报》
CAS
北大核心
2024年第3期1-8,共8页
Journal of Light Industry
基金
河南省重点研发与推广专项项目(242102110100)
郑州轻工业大学基础研究基金资助项目(23XJCYJ018)。
关键词
杜仲叶多糖
植物乳杆菌CICC
20022
胆盐耐受性
Eucommia ulmoides Oliv.leaf polysaccharide
Lactobacillus plantarum CICC 20022
bile salt tolerance
