摘要
黄花菜(Hemerocallis citrina)富含多种营养元素和生物活性成分,是一种传统的食药同源植物。萜类化合物是黄花菜的一类重要的生物活性物质,为了了解黄花菜根、茎、叶、花各器官中的优势萜类代谢物,该研究利用基于超高效液相色谱-串联质谱(ultra performance liquid chromatography-tandem mass spectrometry,UPLC-MS/MS)技术的广靶代谢组学对黄花菜4个器官的萜类代谢物进行分析。共鉴定出萜类物质43种,其中单萜7种、二萜18种、三萜16种、三萜皂苷2种,各占总萜类数的16.2%、41.9%、37.2%、4.7%。主成分分析和聚类分析表明质谱数据可靠,不同器官间的萜类物质积累量差异明显。根中的优势代谢物为异海松二烯酮、海松二烯酮、松香酸、scutebarbolide C、异海松酸;茎中的优势代谢物为3,9-二羟基-13(14)-半日花烯-16,15-内酯、9,19-环羊毛甾-24-烯-3-醇(环阿尔廷醇);叶中的优势代谢物为viteagnusin A、对映-3β-乙酰氧基异海松-15-烯-8β-醇;花中的优势代谢物为牡荆内酯、7-O-coumaroyl-loganic acid。该研究详细列举了黄花菜所有萜类化合物在根、茎、叶、花中的相对含量,为黄花菜各器官萜类化合物生物学功能研究奠定基础,对食品工业和医疗保健具有重要意义。
Hemerocallis citrina is an edible and medicinal homologous plant,which is rich in many nutrients and bioactive components.Terpenoids are a kind of important bioactive component in H.citrina.To understand the dominant terpenoid metabolites in H.citrina roots,stems,leaves,and flowers,respectively,this study analyzed the terpenoid metabolites in these 4 organs based on the ultra performance liquid chromatography-tandem mass spectrometry technology of widely targeted metabolomics.A total of 43 terpenoids were identified,including 7 kinds of monoterpenoids(16.2%),18 kinds of ditepenoids(41.9%),16 kinds of triterpene(37.2%),and 2 kinds of triterpene saponin(4.7%).Principal component analysis and clustering analysis indicated that the mass spectrometry data were reliable,and the accumulation of terpenoids in different organs was significantly different.The dominant terpenoid metabolites in roots included isopimaradieneone,pimaradienone,abietic acid,scutebarbolide C,and isopimaric acid.The dominant terpenoid metabolites in stems included 3,9-dihydroxy-13(14)-labden-16,15-olide and 9,19-cyclolanost-24-en-3-ol(cycloartenol).The dominant terpenoid metabolites in leaves included viteagnusin A and ent-3β-acetoxyisopimar-15-8β-ol.The dominant terpenoid metabolites in flowers included vitexilactone and 7-O-coumaroyl-loganic acid.This study listed the relative contents of all terpenoids in roots,stems,leaves,and flowers of H.citrina in detail,which provided the foundation for further investigation of the different usages of various plant organs of H.citrina,and the information is important for the food industry and medical treatment.
作者
吕虹瑞
武珍珍
洪沙沙
贾慧敏
郭尚
LYU Hongrui;WU Zhenzhen;HONG Shasha;JIA Huimin;GUO Shang(Shanxi Institute for Functional Food,Shanxi Agricultural University,Taiyuan 030031,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2024年第12期299-307,共9页
Food and Fermentation Industries
基金
山西农业大学博士科研启动项目(2021BQ121)
山西省基础研究计划项目(202203021222168)
国家重点研发计划项目(2021YFD1600102)。
关键词
黄花菜
器官
萜类
超高效液相色谱-串联质谱
广靶代谢组学
Hemerocallis citrina
organs
terpenoid
ultra performance liquid chromatography-tandem mass spectrometry
widely targeted metabolomics
