摘要
以分离自广西乐业天坑群的树舌灵芝的深层发酵产物(菌丝体、发酵液)为试材,采用滤纸片法、二倍稀释法,研究了树舌灵芝发酵产物提取物的抑菌作用,以期为开发防腐、食品保鲜产品提供参考依据。结果表明:树舌灵芝菌丝体提取物和发酵液提取物中均含有抗菌活性物质,发酵液乙酸乙酯提取物的抑菌能力最强,对10种供试菌均有较好的抑菌效果;发酵液乙酸乙酯提取物梯度洗脱、分离纯化后得到的30%乙酸乙酯组分(B5)的抑菌能力最高,对10种供试菌的抑菌圈直径基本上均最大;B5对藤黄微球菌的最低抑菌浓度(MIC)值为25 mg·mL^(-1),对大肠杆菌等5种菌的MIC值为50 mg·mL^(-1),对酿酒酵母的MIC值为200 mg·mL^(-1),对黑曲霉、米曲霉和绿色木霉的MIC值为400 mg·mL^(-1)。
The antimicrobial activity of Ganoderma lucidum fermentation products(mycelium and fermentation broth)isolated from Leye Tiankeng Group in Guangxi was studied by using filter paper and double dilution method,in order to provide reference for developing anti-corrosion and food preservation products.The results showed that both mycelium extract and fermentation broth extract contained antibacterial active substances,and ethyl acetate extract of fermentation broth had the strongest antibacterial activity against all 10 tested microbes.The 30%ethyl acetate component(B5)of ethyl acetate extract from fermentation broth had the highest antibacterial activity,and the diameters of antibacterial zone were basically the largest.The minimum inhibitory concentration(MIC)value of B5 against Micrococcus luteus was 25 mg·mL^(-1),the MIC value for Escherichia coli,Bacillus subtilis,Proteus vulgaris,Staphylococcus aureus and Candida albicans were 50 mg·mL^(-1),the MIC value for Saccharomyces cerevisiae was 200 mg·mL^(-1),and the MIC value for Aspergillus niger,Aspergillus oryzae and Trichoderma viride was 400 mg·mL^(-1).
作者
谌斌
孙秋芳
CHEN Bin;SUN Qiufang(Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection,Ministry of Education,Guilin,Guangxi 541004;College of Environment and Resources,Guangxi Normal University,Guilin,Guangxi 541004)
出处
《北方园艺》
CAS
北大核心
2024年第10期99-105,共7页
Northern Horticulture
基金
国家自然科学基金资助项目(41967019)
广西自然科学基金面上资助项目(2011GXNSFA018080)。
关键词
树舌灵芝
深层发酵
提取物
抑菌作用
最低抑菌浓度
Ganoderma applanatum
submerged fermentation
extract
bacteriostasis
minimum inhibitory concentration
