摘要
为有效预防和治疗藏仔猪腹泻,采用PCR和Kirby-Bauer纸片扩散法对西藏林芝市舍饲藏仔猪分离的102株腹泻源大肠杆菌(Escherichia coli)毒力基因、粘附因子基因和耐药性进行检测。大肠杆菌毒力基因和菌毛粘附因子基因检测结果表明,检出率最高的菌毛粘附因子基因是F18(45.10%),其次是F4(15.69%),所有菌株均未检出F41基因;102株菌株中有37株菌株未检出菌毛粘附因子基因。毒力基因中检出率最高的是Stx2e基因(25.49%),其次为STb:EAST-1基因(21.57%)和STa:STb基因(17.65%)。大肠杆菌菌毛粘附因子基因和非菌毛粘附因子基因检测结果表明,在检出AIDA-1基因的菌株中菌毛粘附因子基因(F18、F4)的菌株占比为44.73%,在检出paa基因的菌株中菌毛粘附因子基因(F18、F4)的菌株占比为54.17%;在检出eae基因的菌株中没有检测到F4基因。大肠杆菌对氯霉素、四环素、氨苄西林和链霉素表现出高的耐药性,对3种及以上抗生素产生耐药性的大肠杆菌菌株数占总菌株数的86.27%。
To effectively prevent and treat diarrhea in Tibetan piglets,PCR and Kirby Bauer paper diffusion methods were used,the virulence genes,adhesion factor genes and drug resistance of 102 strains of Escherichia coli from Tibetan piglets with diarrhea in Linzhi,Xizang were detected.The detection results of virulence genes and pili adhesion factor genes in Escherichia coli showed that the pili adhesion factor gene with the highest detection rate was F18(45.10%),followed by F4(15.69%),and no F41 gene was detected in all strains;among the 102 strains,the pili adhesion factor gene was not detected in 37 strains.The gene with the highest detection rate among virulence genes was Stx2e(25.49%),followed by the STb:EAST-1 gene(21.57%)and the STa:STb gene(17.65%).The detection results of Escherichia coli pili adhesion factor genes and non pili adhesion factor genes showed that 44.73% of the strains containing AIDA-1 gene had pili adhesion factor genes(F18,F4),and 54.17% of the strains containing paa gene had pili adhesion factor genes(F18,F4);no F4 gene was detected in the strains that the eae gene was detected.Escherichia coli exhibited high drug resistance to chloramphenicol,tetracycline,ampicillin,and streptomycin,with 86.27% of the total bacterial strains exhibiting resistance to three or more antibiotics.
作者
李龙
谭占坤
李文凤
刘锁珠
LI Long;TAN Zhan-kun;LI Wen-feng;LIU Suo-zhu(College of Animal Engineering,Yangling Vocational&Technical College,Yangling 712100,Shaanxi,China;College of Animal Science,Xizang Agricultural and Animal Husbandry University,Linzhi 860000,Tibet,China)
出处
《湖北农业科学》
2024年第6期156-160,共5页
Hubei Agricultural Sciences
基金
西藏自治区重点研发计划项目(XZ202001ZY0039N)
国家自然科学基金项目(31760673)
杨凌职业技术学院自然科学研究基金项目(A2019066)。
