摘要
以一株怀山药内生毛壳菌Chaetomium sp.L-45为研究对象,对其次生代谢产物进行分离和结构鉴定。对毛壳菌进行大米发酵,通过硅胶柱色谱、Sephadex LH-20、半制备高效液相色谱等分离技术分离发酵物的乙酸乙酯提取物,利用NMR、HR-ESI-MS等波谱解析技术进行化合物的结构鉴定。从目标菌株的乙酸乙酯提取物中分离得到4个化合物,分别鉴定为3,4-二甲氧基-5-羟基-6-丙基-苯甲酸(1)、5-异戊烯基-吲哚-3-羧酸(2)、lecanorin(3)和麦角甾醇(4),其中,化合物1为新化合物,化合物2和3系首次从毛壳菌属真菌中分离得到。
In this study,the secondary metabolites of an endophytic Chaetomium sp.L-45 from Dioscorea opposita were isolated and identified.The ethyl acetate extract of the rice fermentation of the fungus were separated by silica gel column chromatography,Sephadex LH-20,semi-preparative High-performance liquid chromatography,etc.,and the chemical structures of the compounds were identified by NMR and HR-ESI-MS analysis.As a result,four compounds were isolated and identified as 3,4-dimethoxy-5-hydroxy-6-propyl-benzoic acid(1),5-isoprenylindole-3-carboxylic acid(2),lecanorin(3),and ergosterol(4).Among them,compound 1 is a new compound,compounds 2 and 3 were isolated from genus Chaetomium for the first time.
作者
盛蒙珂
赵莹菲
田若丹
文春南
刘淼
麻兵继
SHENG Meng-ke;ZHAO Ying-fei;TIAN Ruo-dan;WEN Chun-nan;LIU Miao;MA Bing-ji(College of Agronomy,Henan Agricultural University;Zhengzhou Key Laboratory of Germplasm Resources Innovation and Metabolic Engineering of Traditional Chinese Medicine,Henan Agricultural University,Zhengzhou 450046,China)
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2024年第6期982-985,962,共5页
Natural Product Research and Development
基金
国家自然科学基金(U1604169)
河南省自然科学基金(232300421127)
河南农业大学博士启动基金(30500662)。
关键词
毛壳菌
次生代谢产物
提取分离
结构鉴定
Chaetomium sp.
secondary metabolites
extraction and isolation
structure elucidation
