摘要
为探究黑土区减氮对大豆光合特性及光合氮利用效率的影响,以传统耕作配合常规施氮为对照处理,设置免耕常规施氮、免耕减氮15%、免耕减氮30%及免耕不施氮共5个处理,研究不同处理对大豆叶面积指数、光合特性、光合-光响应曲线及光合氮利用效率的影响。结果表明,各生育时期大豆净光合速率(Pn)、蒸腾速率(Tr)、气孔导度(Gs)、最大净光合速率(Pn_(max))、暗呼吸速率(R_(d))、表观量子速率(α)、叶绿素含量(SPAD)和叶面积指数(LAI)均随施氮量减少呈先升后降趋势,胞间CO_(2)浓度(Ci)随施氮量减少呈先降后升趋势。与对照处理相比,各生育时期免耕减氮15%处理可显著降低大豆Ci 8.03%~11.16%,提高大豆SPAD、LAI、Pn、Tr、Gs分别为3.21%~6.52%、9.60%~35.00%、14.13%~42.20%、12.93%~38.66%、18.62%~40.87%。免耕减氮15%处理大豆叶片光合-光响应实测曲线变化及光合响应参数显著优于其他处理。不同处理间,各生育时期大豆叶片光合氮利用效率随施氮量减少呈先升后降趋势,其中免耕条件下减氮15%处理较对照提高13.29%~25.43%;叶片光合氮利用效率与LAI、SPAD呈极显著正相关。
In order to investigate the effects of nitrogen reduction on the photosynthetic performance and photosynthetic nitrogen use efficiency of soybean in the black soil region,a control treatment involving conventional nitrogen application under traditional tillage was employed.Five treatments were established:conventional nitrogen application under no-tillage,15%nitrogen reduction under no-tillage,30%nitrogen reduction under no-tillage,and no-tillage without nitrogen application.These treatments aimed to examine and analyze their effects on soybean leaf area index,photosynthetic characteristics,photosynthetic lightresponse curve,and photosynthetic nitrogen use efficiency.Additionally,the study sought to elucidate the mechanism underlying photosynthetic nitrogen use efficiency.The results indicated that the net photosynthetic rate(Pn),transpiration rate(Tr),stomatal conductance(Gs),maximum net photosynthetic rate(Pn_(max)),dark respiration rate(Rd),apparent quantum rate(α),chlorophyll content(SPAD),and leaf area index(LAI)initially increased and then decreased with reduced nitrogen application.With the reduction of nitrogen application,the intercellular CO_(2) concentration(Ci)initially decreased and then increased.Compared with the control treatment,the no-tillage 15%treatment during each growth stage significantly reduced soybean Ci by 8.03%-11.16%and improved soybean SPAD,LAI,Pn,Tr,and Gs by 3.21%-6.52%,9.60%-35.00%,14.13%-42.20%,12.93%-38.66%,and 18.62%-40.87%,respectively.The photosynthesis-light response curve and photosynthetic response parameters of soybean leaves showed significantly improved changes when treated with a 15%nitrogen reduction under no-tillage,compared to other treatments.Additionally,the photo-synthetic nitrogen use efficiency of soybean leaves initially increased and then decreased as the nitrogen application decreased under different treatments.These results indicated the potential benefits of reducing nitrogen application in improving the photosynthetic performance of soybean leaves in a no-tillage system.Compared with the control treatment,the photosynthetic nitrogen use efficiency of soybean leaves increased by 13.29%-25.43%under no-tillage conditions,with the greatest effect observed.Furthermore,there was a significant positive correlation between the photosynthetic nitrogen use efficiency of soybean leaves and LAI as well as SPAD.
作者
刘继龙
曹冉
曹晓强
刘倩倩
董泽
王志卓
LIU Jilong;CAO Ran;CAO Xiaoqiang;LIU Qianqian;DONG Ze;WANG Zhizhuo(School of Water Conservancy and Civil Engineering,Northeast Agricultural University,Harbin 150030,China;Key Laboratory of Effective Utilization of Agricultural Water Resources,Ministry of Agriculture and Rural Affairs,Harbin 150030,China)
出处
《东北农业大学学报》
CAS
CSCD
北大核心
2024年第3期1-9,共9页
Journal of Northeast Agricultural University
基金
国家自然科学基金项目(52079030)
国家重点研发计划项目子课题(2021YFD1500802-2)。
关键词
黑土区
减施氮肥
大豆光合
光合氮效率
免耕
black soil region
nitrogen reduction
soybean photosynthetic
photosynthetic nitrogen efficiency
no-tillage
