摘要
大豆糖蜜含大豆异黄酮、大豆低聚糖、大豆蛋白、蔗糖等,大豆低聚糖主要成分为蔗糖、棉子糖、水苏糖等。为了获得生物净化大豆糖蜜制备功能性大豆低聚糖的菌株,基于从白酒酿造环境中筛选出210株菌株(酵母46株,细菌45株和霉菌119株),以大豆糖蜜中蔗糖保留率、棉子糖保留率、水苏糖保留率及甘露三糖含量为考察指标,对菌株进行初筛和复筛,对复筛菌株进行聚类分析及其生物净化大豆糖蜜中棉子糖和水苏糖特性研究。结果表明,酵母是生物净化大豆糖蜜制备功能性大豆低聚糖的优良菌种;复筛菌株聚类分析表明,第2聚类簇酵母菌,包括酵母YS214、YT112、YT312、YA176、YA281、YT141、YA382、YA133和YT344,处理大豆糖蜜后蔗糖保留率<10%,而棉子糖和水苏糖保留率则>85%,是适合生物净化大豆糖蜜制备功能性大豆低聚糖的菌群。
Soy molasses is rich in soybean isoflavones,soybean oligosaccharides,soybean protein,and sucrose,etc.The main components of soybean oligosaccharides are sucrose,raffinose,stachyose and so on.In order to obtain strains for biopurification of soybean molasses to produce functional soybean oligosaccharides,based on 210 strains(46 yeast strains,45 bacterium strains and 119 mold strains)isolated from Baijiu brewing environment,using the sucrose retention rate,raffinose retention rate,stachyose retention rate,and mannotriose content in soybean molasses as the inspection indexes,the strains were preliminarily screened and rescreened.Cluster analysis and characterization research of raffinose and hydrothreose in biopurification soybean molasses of rescreened strains were carried out.The results showed that yeasts were excellent strains for biopurification soybean molasses to produce functional soybean oligosaccharides.The cluster analysis of rescreened strains showed that cluster 2 yeast strains included yeasts YS214,YT112,YT312,YA176,YA281,YT141,YA382,YA133,and YT344,the sucrose retention rates of them were less than 10%after processing soybean molasses,while the retention rates of raffinose and stachyose were greater than 85%,which were suitable for biopurification soybean molasses to produce functional soybean oligosaccharides.
作者
杨然
仲秀芳
唐艳斌
马景浩
田淑斌
杜亿华
范光森
YANG Ran;ZHONG Xiufang;TANG Yanbin;MA Jinghao;TIAN Shubin;DU Yihua;FAN Guangsen(School of Food and Health,Beijing Technology and Business University,Beijing 100048,China;National Institute for Nutritionand Health,Chinese Center for Disease Control and Prevention,Beijing 100050,China;Sweet Code Nutrition&Health Institute,Zibo 256306,China;Beijing Engineering and Technology Research Center of Food Additives,Beijing Technology andBusiness University,Beijing 100048,China)
出处
《中国酿造》
CAS
北大核心
2024年第6期88-95,共8页
China Brewing
基金
北京市自然科学基金面上项目(6222003)。
关键词
大豆糖蜜
酵母
生物净化
功能性大豆低聚糖
水苏糖
棉子糖
soybean molasses
yeast
biopurification
functional soybean oligosaccharide
stachyose
raffinose
