摘要
目的探讨SOX1和PAX1基因甲基化检测对宫颈高级别病变二次分流的价值。方法采集122例人乳头瘤病毒(HPV)阳性患者的宫颈脱落细胞,同时行液基薄层细胞学检查(TCT)和SOX1/PAX1基因甲基化检测。结果HPV联合TCT检测对宫颈上皮内瘤变(CIN)2级及以上(CIN2+)检出灵敏度为95.24%,特异度为23.75%。结合SOX1/PAX1基因甲基化检测二次分流后,对CIN2+检出灵敏度为83.33%,与HPV联合TCT检测差异无统计学意义(P=0.078);特异度为77.50%,明显高于HPV联合TCT检测(P<0.001)。CIN2+组SOX1/PAX1基因甲基化水平高于正常宫颈组织及CIN1组(P<0.001)。SOX1、PAX1基因甲基化对CIN2+诊断的截断值分别为-11.81、-11.98。结论SOX1/PAX1基因甲基化检测二次分流后,CIN2+检出的效能和准确性明显提升。
Objective To evaluate the value of SOX1 and PAX1 gene methylation detection in the secondary triage of high-grade cervical lesions.Methods Exfoliated cervical cells were collected from 122 patients tested positive for human papilloma virus(HPV)and subjected to thin-prep cytologic test(TCT)and SOX1/PAX1 gene methylation tests.Results The HPV test combined with TCT showed the sensitivity of 95.24%and the specificity of 23.75%for detecting cervical intraepithelial neoplasia(CIN)grade 2 and above(CIN2+).After the addition of the SOX1/PAX1 gene methylation detection in secondary triage,the sensitivity for detecting CIN2+was 83.33%,which had no statistically significant difference from the sensitivity of TCT combined with HPV test(P=0.078).However,the specificity reached 77.50%,which was significantly higher than that of HPV test combined with TCT(P<0.001).The SOX1/PAX1 gene methylation level in the CIN2+group was higher than those in the normal cervical tissue and the CIN1 group(P<0.001).The cut-off values of SOX1 and PAX1 gene methylation for CIN2+detection were-11.81 and-11.98,respectively.Conclusion Adding the detection of SOX1/PAX1 gene methylation in secondary triage significantly improves the efficiency and accuracy of CIN2+detection.
作者
郭艳平
杨青
王诗睿
李思敏
于博阳
杨筱凤
GUO Yanping;YANG Qing;WANG Shirui;LI Simin;YU Boyang;YANG Xiaofeng(Department of Obstetrics and Gynecology,The First Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710061,China)
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
2024年第3期329-333,共5页
Acta Academiae Medicinae Sinicae
基金
陕西省创新能力支撑计划(2023-CX-TD-68)。
