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龙麦系列部分品种(系)Wx-B1b基因检测与淀粉特性分析

Molecular Detection of Wx-B1b Gene and Analysis of Starch Characteristics in Some Varieties(Lines)of Longmai Series
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摘要 小麦品质改良是东北春麦区强筋小麦育种的主要目标之一,小麦籽粒淀粉特性是决定面条品质的重要因素。淀粉特性与直链淀粉含量密切相关,Wx-B1b基因为控制小麦直链淀粉含量的主效基因。为明确该基因在龙麦系列品种(系)中的分布情况,从而为面包面条兼用型强筋小麦育种和品质改良提供有用信息,利用Wx-B1b基因特异性分子标记检测了153份小麦品种(系),并对该基因在东北春麦区强筋小麦遗传背景下淀粉特性的影响进行分析。结果表明,该特异性标记能准确鉴定Wx-B1b基因,在检测的153份材料中,82份材料含有Wx-B1b基因,占比为53.6%;黏度仪的峰值黏度在Wx-B1b和Wx-B1a基因型间差异达到显著水平(P<0.05),为淀粉特性改良的主效基因。因此在东北春麦区为进一步提高面包面条兼用型强筋小麦新品种选育效率,在亲本组配、后代选择及稳定品系处理中应加强Wx-B1b基因的应用和选择。 Wheat quality improvement is one of the great challenges of strong-gluten wheat breeding in the Northeast Spring Wheat Zone.The starch characteristics of wheat grains are important factors determining noodle quality.The starch characteristics are closely related to the content of amylose in wheat.The Wx-B1b gene is the major gene controlling the content of amylose in wheat.In order to clarify the distribution of this gene in the Longmai series varieties(lines)and provide useful information for quality improvement in bread and noodle type strong gluten wheat breeding,153 wheat varieties(lines)were detected by Wx-B1b gene specific molecular markers in this study.And the influence of this gene on starch characteristics in the genetic background of strong gluten wheat in the northeast spring wheat region were analyzed.The results showed that this specific marker can accurately identify the Wx-B1b gene.Among the 153 materials detected,82 materials contained the Wx-B1b gene,accounting for 53.6%.The peak viscosity of the viscometer showed a significant difference(P<0.05)between the Wx-B1b and Wx-B1a genotypes,indicating that it was the main gene for improving starch properties.Therefore,in the spring wheat region of Northeast China,in order to further improve the breeding efficiency of bread and noodle type strong gluten wheat,Wx-B1b gene should be used and selected in parent pairing,off spring selection,and stable strain treatment.
作者 宋维富 杨雪峰 刘东军 赵丽娟 仇琳 宋庆杰 张春利 辛文利 SONG Weifu;YANG Xuefeng;LIU Dongjun;ZHAO Lijuan;QIU Lin;SONG Qingjie;ZHANG Chunli;XIN Wenli(Crop Recourse Institute,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China)
出处 《黑龙江农业科学》 2024年第7期1-5,共5页 Heilongjiang Agricultural Sciences
基金 财政部和农业农村部现代农业产业技术体系春麦改良岗位科学家项目(CARS-03-12) 黑龙江省自然科学基金(YQ2020C039) 黑龙江省博士后科研启动基金(LBH-Q20176) 黑龙江省省属科研院所科研业务费(CZKYF2021-2-B007)。
关键词 小麦 育种 龙麦系列 稳定品系 Wx-B1b基因 wheat breeding Longmai series stable strain Wx-B1b gene
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