摘要
为了了解云南大额牛瘤胃细菌Umcel-2蛋白的生物学特性、结构及功能等特征,阐明大额牛瘤胃细菌Umcel-2蛋白高效降解纤维素的生物学功能,试验采用多种在线软件和工具对Umcel-2蛋白进行生物信息学分析研究。结果表明:Umcel-2蛋白分子式为C1983H2987N541O670S15,相对分子质量为4.56 kDa,属于一个定位于细胞质中,无跨膜区及信号肽的亲水型酸性稳定蛋白;Umcel-2蛋白含有1个保守区域pfam00150且属于糖苷水解酶家族5,二级结构由α-螺旋、无规则卷曲发挥主要功能,三级结构同源建模质量高且与芳基磷酸化-β-D-葡萄糖苷酶BglC相似;Umcel-2蛋白存在3个N端糖基化位点和49个磷酸化位点,且其活性位点由甘氨酸、丝氨酸、苏氨酸、亮氨酸和天冬酰胺等氨基酸组成,有利于提高其纤维素酶活性。系统进化树结果表明,Umcel-2蛋白与未培养生物AEX97596.1纤维素酶的纤维素酶蛋白高度同源,表现出内切葡聚糖酶和β-葡萄糖苷酶的纤维素酶活。研究表明,生物信息学方法预测瘤胃细菌Umcel-2蛋白具有较高的纤维酶活性,为瘤胃细菌高效降解纤维素的深入研究提供了参考。
This study was aimed to understand the biological characteristics,structure and function of Umcel-2 protein in rumen bacteria of Yunnan Gayal(Bas frontalis),and to elucidate the biological function of Umcel-2 protein in efficient degradation of cellulose in rumen bacteria of Gayal.Varieties of online software and tools were used for bioinformatics analysis of Umcel-2 protein.The results showed that the Umcel-2 protein with a molecular formula of C1983H2987N541O670S15 and a relative molecular mass of 4.56 kDa,which was a hydrophilic acidic stable protein localized in the cytoplasm without transmembrane regions and signal peptides.Umcel-2 protein had one conserved region pfam00150 and belonged to the glycoside hydrolase family 5,its secondary structure was mainly composed ofα-helix and irregular curling,and its tertiary structure was of high homologous modeling quality and similar to that of aryl phosphoryl-β-Dglucosidase BglC.The Umcel-2 protein had three N-terminal glycosylation sites and 49 phosphorylation sites,and its active sites were composed of amino acids,such as glycine,serine,threonine,leucine and asparagine,which were conducive to improving its cellulase activity.The Phylogenetic tree analysis results showed that Umcel-2 protein was highly homologous to the cellulase protein of uncultured AEX97596.1 cellulase,showing the cellulase activity of endoglucanase andβ-glucosidase.The study indicates that the bioinformatics analysis results predicts that the Umcel-2 protein of rumen bacterial had high cellulase activity,providing a reference for the in-depth study of the efficient cellulose degradation by rumen bacteria.
作者
郑洁怡
周子健
杨舒黎
李川
ZHENG Jie-yi;ZHOU Zi-jian;YANG Shu-li;LI Chuan
出处
《饲料研究》
CAS
北大核心
2024年第10期82-86,共5页
Feed Research
基金
国家自然科学基金项目(项目编号:32060762)
广东省教育厅重点领域专项(科技服务乡村振兴)(项目编号:2022ZDZX4041)。
