摘要
油茶象甲Curculio chinensis Chevrolat是危害中国特有木本油料树种——油茶的专性蛀果害虫,也是中国林业危险性有害生物之一,在中国不同油茶产区均有分布,对油茶的为害呈加重趋势。为了深入研究其遗传基础和寄主适应性,确定适合鞘翅目象甲科基因组测序研究策略,首先采取Survey测序,之后做深度基因组测序和大规模种群测序。研究采用二代高通量测序技术(MGISEQ-2000),测定了油茶象甲基因组大小,并估计该物种基因组的杂合度、重复率和GC含量。结果显示:油茶象甲基因组大小约为1356.82 Mb,测序深度达到50×;K-mer分析油茶象甲基因组虽然具有杂合峰,但杂合率较低,为1.20%;基因组重复序列含量约为77%。该研究结果对于揭示油茶象甲适应性进化具有重要意义,可为后续构建完整基因组和多种群基因组测序提供策略选择依据。
The camellia weevil,Curculio chinensis Chevrolat,is a host-specific predator of the seeds of oil-tea camellia,endemic woody oil-bearing plant in China.It is one of the dangerous pests in Chinese forestry,and is widely distributed in different oil-tea camellia planting areas of China with increasing damage.In order to further study its genetic basis and host adaptability,and determine sequencing strategies that were suitable for genome sequencing of Curculionidae in Coleoptera,survey sequencing was first adopted to lay the foundation for further deep genome sequencing and large-scale population sequencing.In this study,reads from secondgeneration high-throughput sequencing technology(MGISEQ-2000 Pair-End sequencing platform)was used to determine the genome size of C.chinensis,and estimate the heterozygosity,repetition rate and GC content of the genome mass of this species.The results showed that the genome size of C.chinensis was about 1356.82 Mb,and the sequencing depth reached 50×.K-mer analysis showed that although there was a heterozygous peak in the C.chinensis genome,the heterozygous rate was as low as 1.20%.And the content of repeated sequences was about 77%.The results of this study are of great significance to reveal the adaptive evolution of Camellia weevil,and can provide a basis for the selection of strategies for the subsequent construction of a complete genome and multi-population genome sequencing.
作者
张丽
吴佳茜
曾萝琦
刘惠
汤小宇
ZHANG Li;WU Jiaxi;ZENG Luoqi;LIU Hui;TANG Xiaoyu(Jiujiang University/Institute of Jiangxi Oil-tea Camellia,Jiujiang,Jiangxi 332005)
出处
《中国农学通报》
2024年第17期135-141,共7页
Chinese Agricultural Science Bulletin
基金
江西省教育厅科技项目“油茶象甲种群遗传结构及局域适应性进化研究”(GJJ201823)。
