摘要
为评价第二代抗凝血类灭鼠剂溴敌隆的潜在生态毒性效应,以模式动物斑马鱼为研究对象,研究了溴敌隆对斑马鱼胚胎/仔鱼的发育毒性以及对抗氧化系统的影响。研究结果表明,当溴敌隆浓度高于0.39 mg·L^(-1)时,斑马鱼胚胎/仔鱼死亡率及畸形率显著升高,与对照组相比具有显著性差异,斑马鱼仔鱼体内的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性以及还原型谷胱甘肽(GSH)和丙二醛(MDA)的含量随溴敌隆浓度增大,均呈逐渐升高趋势,并在1.18 mg·L^(-1)浓度组达到最大值,具有显著差异,表明溴敌隆对斑马鱼仔鱼的抗氧化系统造成严重损伤,从而导致机体死亡。对溴敌隆暴露导致的斑马鱼胚胎/仔鱼的发育毒性以及氧化损伤的机制进行了初步探讨,为进一步研究溴敌隆对动物的发育毒性机制以及其潜在的生态危害提供了研究基础。
To investigate the potential ecological toxicity of the second generation anticoagulant rodenticide bromadiolone,the developmental toxicity of bromadiolone on zebrafish embryos/larvae and its effect on the antioxidant system were studied.The results showed that the exposure to bromadiolone could lead to developmental toxicity such as death and deformity in zebrafish embryos/larvae.When the bromadiolone concentration was higher than 0.39 mg·L^(–1),the mortality and deformity rate of zebrafish embryos/larvae increased significantly.Exposure to bromadiolone also had a significant effect on the antioxidant system of zebrafish embryos/larvae.The activities of SOD and CAT and the contents of GSH and MDA increased significantly with the increase of bromadiolone exposure concentration,indicating that bromadiolone caused oxidative damage to zebrafish embryos/larvae,which led to the attenuation of other body functions and even death.In this paper,the developmental toxicity and oxidative damage of embryos/larvae caused by bromadiolone exposure were investigated and discussed,which provided a basis for further study on the developmental toxicity mechanism of bromadiolone on animals and its potential ecological hazards.
作者
吴元钊
徐帆
张安慧
应剑波
姚伟宣
王继业
王斌杰
WU Yuanzhao;XU Fan;ZHANG Anhui;YING Jianbo;YAO Weixuan;WANG Jiye;WANG Binjie(Zhejiang Police College,Key Laboratory of Drug Prevention and Control Technology of Zhejiang Province,Hangzhou 310051,China;Institute of Criminal Science and Technology Public Security Bureau of Hangzhou,Hangzhou 310004,China)
出处
《生态科学》
CSCD
北大核心
2024年第2期177-182,共6页
Ecological Science
基金
国家重点研发计划项目课题(2018YFC0807201)
浙江省重点研发计划项目(2021C03135)
杭州农业与社会发展科研主动设计项目(20190101A08)
国家级大学生创新创业训练计划项目(2202111483024)。
关键词
溴敌隆
发育毒性
氧化损伤
斑马鱼
bromadiolone
developmental toxicity
oxidative damage
zebrafish
