摘要
目的 基于抑菌药效优化复方铁苋最佳配伍及提取工艺,并揭示该中药复方的抑菌物质。方法 以抗嗜水气单胞菌药效为考察指标筛选复方铁苋最佳配伍,以没食子酸的含量和抑菌活性为评价指标,通过单因素实验和响应面法优化复方铁苋的提取工艺;对不同提取工艺的复方铁苋建立化学指纹图谱并指认“共有峰”,通过相关分析建立指纹图谱与抑菌药效间的“谱效关系”,并采用UPLC-Q-TOF-MS技术进行成分鉴定,找出抑制嗜水气单胞菌的关键成分。结果 复方铁苋以铁苋菜∶五倍子∶诃子=2∶2∶1(w/w/w)为最佳配伍,以料液比为1∶25,浸泡22 min后煎煮60 min为最佳提取工艺,该条件下复方铁苋提取物对嗜水气单胞菌具有最佳抑制效果;从复方铁苋指纹图谱中找出4个与抑菌活性呈高度正相关的物质:诃子酸>诃子鞣酸>没食子酸-4-O-(6-O-没食子酰)葡萄糖苷>没食子酸(相关系数在0.499~0.846之间)。结论 铁苋菜、五倍子、诃子在最佳配伍及工艺条件下具有明显抑制嗜水气单胞菌的活性,通过“谱效关系”快速找出该中药复方中潜在的抑菌物质,为复方铁苋进一步开发为防治嗜水气单胞菌的药物提供科学依据。
Objective To optimize the optimal combination and extraction process of Fufang Tiexian based on its antibacterial efficacy,and to reveal the antibacterial substances of this traditional Chinese medicine compound.Methods The optimal combination of Fufang Tiexian was selected based on the efficacy of anti Pseudomonas aeruginosa as the evaluation index,and the content of gallic acid and antibacterial activity were used as the evaluation index.The extraction process of Fufang Tiexian was optimized through single factor experiments and response surface methodology;Establish chemical fingerprints of Fufang Tiexian with different extraction processes and identify“common peaks”.Through correlation analysis,establish a“spectrum effect relationship”between the fingerprint and antibacterial efficacy,and use UPLC-Q-TOF-MS technology for component identification to identify the key components that inhibit Aeromonas hydrophila.Results The optimal combination of Fufang Tiexian was found to be Acalypha australis Linn.∶gallnut∶Terminalia chebula Retz.=2∶2∶1(w/w/w),with a solid-liquid ratio of 1∶25.The optimal extraction process was to soak for 22 minutes and then boil for 60 minutes.Under these conditions,the extract of Fufang Tiexian had the best inhibitory effect on Aeromonas hydrophila;Four substances highly positively correlated with antibacterial activity were identified from the fingerprint of Fufang Tiexian∶chebulic acid>chebulic acid>gallic acid 4-O-(6-O-gallic acid)glucoside>gallic acid(correlation coefficient between 0.499 and 0.846).Conclusion Acalypha australis Linn,Chinese gallnut,and Terminalia have significant inhibitory activity against Pseudomonas aeruginosa under the optimal combination and process conditions.Through the“spectrum effect relationship”,potential antibacterial substances in this traditional Chinese medicine compound can be quickly identified,providing scientific basis for the further development of Fufang Tiexian as a drug for the prevention and treatment of Pseudomonas aeruginosa.
作者
陈紫莹
黄姣丽
廖映红
陈佳婷
孙旭高
陈星
肖遂
CHEN Ziying;HUANG Jiaoli;LIAO Yinghong;CHEN Jiating;SUN Xugao;CHEN Xing;XIAO Sui(School of Forestry and Landscape Architecture,South China Agricultural University,Guangzhou 510642,China)
出处
《现代中医药》
CAS
2024年第4期100-108,共9页
Modern Chinese Medicine
基金
广东省自然科学基金面上项目(2021A1515012153)。
关键词
铁苋菜
五倍子
诃子
UPLC-Q-TOF-MS
嗜水气单胞菌
工艺优化
谱效关系
Acalypha australis Linn.
gallnut
Terminalia chebula Retz.
UPLC-Q-TOF-MS
Aeromonas hydrophila
Process optimization
Spectral effect relationship
