摘要
鳗弧菌(Vibrio anguillarum)是水产养殖中常见病原菌之一。为探究鳗弧菌感染海洋经济动物单环刺螠(Urechis unicinctus)后宿主基因表达水平的变化和应答细菌感染的分子机制,运用高通量测序技术对感染鳗弧菌的单环刺螠对照组、易感组和抗感组体腔细胞进行了转录组测序,并对比分析其差异表达基因(DEGs)及信号通路。结果显示,鳗弧菌感染单环刺螠后,易感组体腔细胞差异表达基因数目为2309个,其中1451个基因表达上调,858个基因表达下调;抗感组中DEGs数目为1955个,其中1311个基因表达上调,644个基因表达下调。KEGG通路富集分析显示,与免疫相关的通路主要有MAPK信号通路、过氧化物酶体、内吞作用、溶酶体、氨基酸生物合成等,筛选出的潜在抗感基因主要有牛磺酸分解代谢双加氧酶(Taurine catabolism dioxygenase,TauD)、细胞色素P450(Cytochrome P450,CYP450)、Casitas B细胞淋巴瘤B(Casitas B lymphoma-b,Cbl-b)、肌动蛋白相关蛋白3(Actin-related protein 3,ACTR3)、热休克蛋白70(Heat shock protein 70,HSP70)、氨基酸通透酶(Amino acid permease,AAPs)、编码葡萄糖激酶(Glucokinase,GCK)、乙醛酸和羟基丙酮酸还原酶(Glyoxylate reductase/hydroxy pyruvate reductase,GRHPR)、胱硫醚β-合酶(Cystathionine beta-synthase,CBS)、组氨酸磷酸酶(Histidine phosphatase,LHPP)、谷草酰乙酸转氨酶2(Glutamic-oxaloacetic transaminase 2,GOT2)、乳酸脱氢酶(Lactate dehydrogenase,LDH)和细胞分裂周期42(Cell division control protein 42,CDC42),易感基因主要有ADP核糖激化因子(ADP-ribosylation factor,ARF)、细胞粘附素(Cytohesin,CYTH)、EH结构域蛋白1(EH domain-containing protein 1,EHD1)、Ras相关蛋白Rab-8A(Ras-related protein Rab-8A,Rab8A)、钙网蛋白(Calreticulin,CALR)和Ras相关的C3肉毒素底物1(Ras-related C3 botulinum toxin substrate 1,RAC1)。实验结果可为鳗弧菌对水产养殖动物的感染机制及单环刺螠对病原菌的免疫应答研究提供数据参考。
Vibrio anguillarum is one of the common pathogenic bacteria in aquaculture.In order to investigate the changes of host gene expression level and the molecular mechanism of response to bacterial infection after V.anguillarum infected marine economic animal Urechis unicinctus,the high-throughput sequencing technology was adopted to sequence the transcriptomes of coelomocytes of different groups of U.unicinctus after infected with V.anguillarum,including control group,susceptibility group and disease-resistance group,and then the differently expressed genes(DEGs)and signal channels were comparative analyzed in this study.The results showed that the number of DEGs in the susceptible group was 2309,including 1451 up-regulated genes and 858 down-regulated genes,and the number of DEGs in the disease-resistance group was 1955,including 1311 up-regulated genes and 644 down-regulated genes,compared with the control group.The KEGG pathway enrichment analysis showed that the immune-related pathways mainly included MAPK signaling pathway,peroxisome,endocytosis,lysosome and amino acid biosynthesis,and the potential resistance genes mainly included Taurine catabolism dioxygenase(TauD),Cytochrome P450(CYP450),Casitas B lymphoma-b(Cbl-b),Actin-related protein 3(ACTR3),Heatshock protein 70(HSP70),Amino acid permease(AAPs),Glucokinase(GCK),Glyoxylate reductase/hydroxypyruvate reductase(GRHPR),Cystathionine beta-synthase(CBS),Histidine phosphatase(LHPP),Glutamic-oxaloacetic transaminase 2(GOT2),Lactate dehydrogenase(LDH),Cell division control protein 42(CDC42),while the susceptibility genes mainly included ADP-ribosylation factor(ARF),Cytohesin(CYTH),EH domain-containing protein 1(EHD1),Ras-related protein Rab-8A(Rab8A),Calreticulin(CALR)and Rasrelated C3 botulinum toxin substrate 1(RAC1).This study may provide data referenced for further exploring the mechanism of infection against aquaculture animals by V.anguillarum and the immune response of U.unicinctus to pathogenic bacteria.
作者
王思婕
许星鸿
涂丁颖
刘顺
李百玉
朱龙
万文杰
WANG Sijie;XU Xinghong;TU Dingying;LIU Shun;LI Baiyu;ZHU Long;WAN Wenjie(School of Marine Science and Fisheries,Jiangsu Ocean University,Lianyungang 222005,China;Lianyungang Jiehao Aquatic Products Co.,Ltd.,Lianyungang 222000,China)
出处
《江苏海洋大学学报（自然科学版）》
CAS
2024年第2期5-16,共12页
Journal of Jiangsu Ocean University:Natural Science Edition
基金
江苏省科技厅苏北科技专项(SZ-LYG202124,LYG-SZ201814)
连云港市花果山英才计划(KK20100)
连云港市科技局现代农业项目(CN1411)
江苏海洋大学研究生科研与实践创新计划项目(KYCX2023-137)。
关键词
鳗弧菌
单环刺螠
转录组
免疫应答
Vibrio anguillarum
Urechis unicinctus
transcriptome
immune response
