摘要
铁皮石斛为我国传统的药用和食用同源的植物,具有极高的中药价值,尤其是其多糖含量最为显著。本研究以山西产吸水石栽培的铁皮石斛为原材料,通过单因素及响应面分析法优化低共熔溶剂提取铁皮石斛多糖工艺,然后分析不同浓度多糖对DPPH自由基、羟基自由基和ABTS+自由基的清除效果。结果显示,低共熔溶剂提取铁皮石斛多糖工艺:采用L-脯氨酸-草酸低共熔溶剂体系,在料液比1∶21(g/mL)、温度56℃的条件下超声30 min,最终多糖得率为(34.35±0.2)%。进行抗氧化实验分析,发现5 mg/mL的多糖浓度对DPPH自由基、羟基自由基和ABTS自由基的清除率最高,达到37.7%、96.6%和77.8%。本研究为铁皮石斛多糖的提取提供了一种有效的方法,同时探究了多糖添加量对抗氧化能力的变化,为铁皮石斛多糖的提取和抗氧化添加剂的开发提供思路。
Dendrobium officinale is a traditional plant in China used for both medicinal and edible purposes,with high traditional Chinese medicinal value,especially due to its significant polysaccharide content.Dendrobium officinale cultivated on water-absorbing stones in Shanxi province was taken as the raw materials,the polysaccharide extraction process was optimized using eutectic solvent extraction technique through single-factor and response surface analysis.Subsequently,the scavenging effects of different concentrations of polysaccharides on DPPH,hydroxyl radicals,and ABTS+were analyzed.The results showed that the optimized polysaccharide extraction process of Dendrobium officinale using a L-proline-oxalic acid eutectic solvent system was as follows:with a material-to-liquid ratio of 1∶21(g/mL),the temperature of 56℃,under ultrasound for 30 minutes,resulted in a polysaccharide yield of(34.35±0.2)%.Antioxidant experiments revealed that a polysaccharide concentration of 5 mg/mL exhibited the highest scavenging rates for DPPH,hydroxyl radicals,and ABTS+,reaching 37.7%,96.6%,and 77.8%,respectively.This study provides an effective method for the extraction of polysaccharides from Dendrobium officinale,and explores the impact of polysaccharide concentration on antioxidant capacity,offering insights for the extraction of polysaccharides from Dendrobium officinale and the development of antioxidant additives.
作者
侯天宇
杨景瑶
胡扬凡
张芷瑜
郭海云
李河
张志军
HOU Tianyu;YANG Jingyao;HU Yangfan;ZHANG Zhiyu;GUO Haiyun;LI He;ZHANG Zhijun(College of Chemistry and Chemical Engineering,North University of China,Taiyuan 030051;Shanxi Lingyun Biotechnology Co.,Ltd.,Jiexiu 032000)
出处
《中国食品添加剂》
CAS
2024年第6期8-15,共8页
China Food Additives
基金
山西省基础研究计划青年项目(202203021222032)
山西省重点研发计划项目特优农业技术创新专项(2022ZDYF123)
关键词
铁皮石斛
多糖
低共熔溶剂法
提取工艺
抗氧化活性
Dendrobium officinale
polysaccharide
low eutectic solvent method
extraction process
antioxidant activity
