摘要
为了探讨聚乙二醇(PEG)渗透胁迫对绿豆萌发的影响,明确干旱胁迫条件下绿豆萌发期的相关各项指标,筛选出抗旱性绿豆品种(系),从而为在绿豆抗旱新品种的筛选过程中提供理论依据,以18个绿豆品种(系)为研究对象,采用浓度为20%的PEG-6000溶液模拟干旱胁迫,对其萌发指标进行测定和分析,并对参试品种的抗旱性采用主成分分析和隶属函数方法进行鉴定和评价。结果表明,与对照相比,20%PEG渗透胁迫绿豆种子后,种子萌发的相关指标都有不同程度的下降,不同品种之间表现出一定的差异性。对6个抗旱性指标进行主成分分析,结果显示,累计贡献率为89.55%,可以有效地反映各数据的变化。18个绿豆品种(系)的综合评价D值为0.389~0.858,依据D值将其聚类分析分为3类,筛选出6个耐旱性品种:晋绿9号、同123-33、同绿5号、同118-6、同绿6号、黄荚绿。通过对18个绿豆品种的抗旱性综合评价,最终得到6个强耐旱品种,3个耐旱品种,9个较敏感品种。萌发期鉴定结果可作为衡量作物抗旱性强弱的一个重要指标,在此基础上,可以进一步结合全生育期的抗旱性评价结果进行综合分析。
In order to explore the effect of polyethylene glycol(PEG) osmotic stress on mung bean germination,clarify the relevant indicators of the mung bean germination under drought stress conditions,screen the drought resistant varieties(lines) of mung bean,and provide a theoretical basis for the screening process of new drought resistant mung bean varieties,in this study,taking 18 mung bean varieties(lines) as the research objects,a 20% of PEG-6000 solution was employed to simulate drought stress,and the germination indicators of the 18 mung bean varieties(lines) were measured and analyzed.The drought resistance of the tested varieties was identified and evaluated using principal component analysis and membership function methods.The results indicated that,compared with the control group,after subjecting mung bean seeds to 20% of PEG osmotic stress,the relevant indicators for seed germination varied in their degrees of decrease,with differences observed among the different varieties.Principal component analysis was performed on six drought resistance indicators,yielding a cumulative contribution rate of 89.55%,effectively capturing the data changes.The comprehensive evaluation of the 18 mung bean varieties(lines)resulted in a D value range of 0.389 to 0.858.Through cluster analysis based on the D value,the varieties were categorized into three groups.Six drought-tolerant varieties were screened,and they were Jinlü 9,Tong123-33,Tonglü 5,Tong118-6,Tonglü 6,and Huangjialü.Following a comprehensive assessment of the drought resistance of the 18 mung bean varieties,6were classified as strongly drought-tolerant varieties,3 as drought-tolerant varieties,and 9 as more sensitive varieties.It should be noted that the identification results during the germination served as an important indicator to measure crop drought resistance,based on which comprehensive analysis could be conducted in further conjunction with the evaluation results for drought resistance throughout the entire growth period.
作者
王桂梅
邢宝龙
刘支平
WANG Guimei;XING Baolong;LIU Zhiping(Institute of High Latitude Crops,Shanxi Agricultural University,Datong 037008,China;Shanxi Agricultural University,Taiyuan 030031,China)
出处
《山西农业科学》
2024年第4期51-57,共7页
Journal of Shanxi Agricultural Sciences
基金
国家重点研发计划子课题(2021YFD1600605-07,2021YFD1600602-07)
财政部和农业农村部:国家现代农业产业技术体系(CARS-08-Z5)
山西农业大学生物育种工程项目(YZGC077)。
关键词
PEG渗透胁迫
绿豆
抗旱性评价
主成分分析
隶属函数方法
PEG osmotic stress
mung bean
drought resistance evaluation
principal component analysis
membership func‐tion methods
