摘要
采用一锅水热法合成的Fe-Co普鲁士蓝类似物(PBAs)作为类过氧化物酶,基于H_(2)O_(2)与3,3',5,5'-四甲基联苯胺(TMB)的显色反应和GSH对氧化TMB(ox-TMB)的还原反应,建立一种用于H_(2)O_(2)和GSH定量分析的比色传感器.在H_(2)O_(2)为2.0~50μmol/L和GSH为6.6~50.63μmol/L范围内均表现出良好的线性关系,检测限分别为0.36和0.32μmol/L.
Fe-Co Prussian blue analogs(PBAs)synthesized by a one-pot hydrothermal method were used as the peroxidase-like enzyme.Based on the color reaction of H_(2)O_(2)with 3,3',5,5'-tetramethylbenzidine(TMB)and the reduction reaction of glutathione(GSH)with oxidized TMB(ox-TMB),a colorimetric sensor for the quantitative analysis of H_(2)O_(2)and GSH was developed.The colorimetric sensor showed a favorable linear relationship in the range of 2.0-50μmol/L for H_(2)O_(2)and 6.6-50.63μmol/L for GSH,and the detection limits were 0.36 and 0.32μmol/L,respectively.
作者
叶佳佳
张武翔
倪建聪
胡世荣
YE Jiajia;ZHANG Wuxiang;NI Jiancong;HU Shirong(College of Chemistry,Chemical Engineering and Environment,Minnan Normal University,Zhangzhou,Fujian 363000,China;College of Environment and Chemical Engineering,Jiangsu University of Science and Technology,Zhenjiang,Jiangsu 212003,China)
出处
《闽南师范大学学报（自然科学版）》
2024年第2期52-63,共12页
Journal of Minnan Normal University：Natural Science
基金
福建省自然科学基金项目(2019J01745)。
