摘要
目的观察眠安方对抑郁症睡眠障碍大鼠血清白细胞介素-6(Interleukin-6,IL-6)、白介素-1β(Interleukin-1β,IL-1β)、肿瘤坏死因子-α(Tumor necrosis factor alpha,TNF-α)及海马脑源性神经营养因子(Brain-derived neurotrophic factor,BDNF)蛋白表达的影响。方法将清洁级雌性大鼠48只按随机数字表法分为模型组、眠安方组、黛力新组、空白组,每组各12只。除空白组外,其他组采用孤养结合慢性温和不可预知应激加快速动眼相睡眠剥夺方法,建立大鼠抑郁症睡眠障碍(Sleep deprivation depression,SDD)模型。采用体质量观察、糖水偏爱实验、旷场实验检测各组大鼠行为学变化指标。酶联免疫吸附测定法(Enzyme-linked immunosorbent assay,ELISA)测定各组大鼠血清IL-6、IL-1β及TNF-α的含量;蛋白质免疫印迹(Western blot,WB)法检测大鼠海马BDNF蛋白表达。结果行为学测定:造模前各组大鼠体质量、糖水偏爱率、旷场实验得分均无差异(P>0.05);造模及给药后,与空白组比较,模型组、眠安方组、黛力新组大鼠体质量、糖水偏爱率、旷场实验得分均明显下降(P<0.05);与模型组比较,眠安方组、黛力新组大鼠体质量、糖水偏爱率、旷场实验得分均升高(P<0.05)。ELISA法检测结果:大鼠血清IL-6、IL-1β及TNF-α含量与空白组比较,模型组显著升高(P<0.01);与模型组比较,眠安方组、黛力新组均显著降低(P<0.01);与眠安方组比较,黛力新组大鼠血清IL-6、IL-1β的含量组间无差异、TNF-α的含量降低(P<0.05)。WB法检测结果:与空白组比较,模型组大鼠海马BDNF蛋白表达水平降低(P<0.05);与模型组比较,眠安方组、黛力新组海马BDNF蛋白表达水平升高(P<0.05);与眠安方组比较,黛力新组大鼠海马BDNF蛋白表达水平升高(P<0.05)。结论眠安方可改善SDD模型大鼠的抑郁行为和睡眠障碍,其作用机制可能与降低血清IL-6、IL-1β及TNF-α的含量,上调海马组织BDNF蛋白的含量有关。
Objective To observe the effects of Mianan Fang on serum expression levels of interleukin-6(IL-6),interleukin-1β(IL-1β),tumor necrosis factor-alpha(TNF-α),and brain-derived neurotrophic factor(BDNF)in rats with depression-related sleep disorders.Methods A total of 48 specific pathogen-free(SPF)healthy female rats were divided into a model group,a Mianan Fang-treated group,a Deanxit-treated group,and a blank group using a random number table.Except for the blank group,the other groups were treated with isolation and chronic unpredictable mild stress to accelerate rapid eye movement sleep deprivation to establish the sleep deprivation depression(SDD)rat model.Behavioral changes of rats in each group were detected by body weight,sucrose preference test,and open field test to determine whether the SDD model was successfully replicated and to verify the therapeutic effect of Mianan Fang.Enzyme-linked immunosorbent assay(ELISA)was used to measure serum levels of IL-6,IL-1β,and TNF-α in rats in each group,while Western blot(WB)was used to detect the expression of BDNF protein in the hippocampus of rats.Results Behavioral measurement:Before modeling,there were no differences in body weight,sucrose preference rate,and open field test scores among the groups(P>0.05).After modeling and administration,compared with the blank group,the body weight,sucrose preference rate,and open field test scores of rats in the model group,Mianan Fang group,and Deanxit group all significantly decreased(P<0.05);compared with the model group,the body weight,sucrose preference rate,and open field test scores of rats in the Mianan Fang group and Deanxit group all increased significantly(P<0.05).ELISA results:Compared with the blank group,the serum level of IL-6,IL-1β and TNF-α in rats in the model group increased significantly(P<0.01);compared with the model group,the levels of IL-6,IL-1β and TNF-α in Mianan Fang group and the Deanxit group decreased significantly(P<0.01);there was no difference in the levels of IL-6 and IL-1β between Deanxit group and Mianan Fang group,but the level of TNF-αin the Deanxit group was lower than Mianan Fang group(P<0.05).WB results:Compared with the blank group,the expression of BDNF protein in the hippocampus of rats in the model group decreased(P<0.05);compared with the model group,the expression of BDNF protein in the Mianan Fang group and the Deanxit group increased(P<0.05);compared with the Mianan Fang group,the expression of BDNF protein in Deanxit group increased(P<0.05).Conclusion Mianan Fang can improve depressive behavior and sleep disorders in SDD model rats,and its mechanism of action may be related to reducing the levels of serum IL-6,IL-1β,TNF-α and up-regulating the expression levels of BDNF protein in the hippocampus.
作者
韩艳丽
孙玉信
HAN Yan-li;SUN Yu-xin(Third Affiliated Hospital of Henan University of Traditional Chinese Medicine,Zhengzhou Henan 450000)
出处
《世界中西医结合杂志》
2024年第6期1139-1144,共6页
World Journal of Integrated Traditional and Western Medicine
基金
河南省中医药科学研究专项课题(20-21ZY2126)。
