摘要
利用生物信息学方法对铁皮石斛GH3基因家族成员进行筛选、鉴定,对其基因结构、蛋白理化性质、亚细胞定位、系统进化、蛋白质二级结构及跨膜预测、基序及染色体定位进行分析,同时分析GH3基因家族在不同组织以及低磷与正常磷水平下的表达模式。在铁皮石斛全基因组中筛选得到13个GH3家族基因,其中一个基因存在可变剪切,表达为14个GH3蛋白。GH3基因家族蛋白可分为3个亚家族,各亚家族中不同基因相对保守;各成员均含有内含子与外显子,内含子相位在0~2,13个基因分布在8染色体上。GH3基因家族在铁皮石斛的8个组织中均有表达,其中白根和花蕾表达量高,茎和唇瓣低,推测GH3基因家族可能参与铁皮石斛根系生长以及开花现蕾。低磷与正常磷水平表达结果表明,低磷水平下叶中基因LOC 110110392、LOC 110100144表达上调,根中基因LOC 110095762、LOC 110107269表达上调。该研究对铁皮石斛糖苷水解酶GH3基因家族成员生物信息学、在不同组织以及正常磷与低磷条件下的表达水平进行研究分析,筛选响应低磷信号的糖苷水解酶基因,为后期研究糖苷水解酶参与低磷条件下铁皮石斛多糖代谢调节机制提供研究基础。
Bioinformatics methods were used to screen and identify the members of the GH3 gene family of Dendrobium officinale,and analyze their gene structure,protein physical and chemical properties,subcellular localization,phylogeny,protein secondary structure and transmembrane prediction,motifs,and chromosome localization.We analyzed the expression patterns of the GH3 gene family in different tissues and under low and normal phosphorus levels.Thirteen GH3 family genes were screened from the genome of D.officinale,with one gene exhibiting variable splicing and expressing 14 GH3 proteins.GH3 gene family proteins can be divided into three subfamilies,in which different genes are relatively conservative.Each member gene contains intron and exon,the phase of intron is between 0 and 2,and 13 genes are distributed on chromosome 8.The GH3 gene is expressed in all 8 tissues of D.officinale,with high expression levels in white roots and flower buds,and low expression levels in stems and lips.It is speculated that the GH3 gene may be involved in the root growth and flowering stage of D.officinale.The expression results of low and normal phosphorus levels showed that under low phosphorus levels,the expression of genes LOC 110110392 and LOC 110100144 in leaves was upregulated,while the expression of genes LOC110095762 and LOC110107269 in roots was upregulated.The bioinformatics of the members of the glycoside hydrolase GH3 gene family of D.officinale,the expression level in different tissues and under normal phosphorus and low phosphorus conditions were studied and analyzed,and the glycoside hydrolase genes responding to low phosphorus signals were screened,providing a research basis for the further study of glycoside hydrolase involved in the regulation mechanism of polysaccharide metabolism in D.officinale under low phosphorus conditions.
作者
李亚萍
金福来
黄宗贵
张涛
段晓婧
姜武
陶正明
陈家栋
LI Yaping;JIN Fulai;HUANG Zonggui;ZHANG Tao;DUAN Xiaojing;JIANG Wu;TAO Zhengming;CHEN Jiadong(Zhejiang Institute of Subtropical Crops,Wenzhou 325005,Zhejiang,China;Wenzhou Jinfulai Agricultural Science and Technology Co.,Ltd.,Wenzhou 325005,Zhejiang,China;Wenzhou Seed Station,Wenzhou 325052,Zhejiang,China;Wenzhou Agricultural Technology Promotion Center,Wenzhou 325052,Zhejiang,China)
出处
《浙江农业学报》
CSCD
北大核心
2024年第4期790-799,共10页
Acta Agriculturae Zhejiangensis
基金
国家自然科学基金(32102485)
温州市农业新品种选育协作组项目(2019ZX003-4)
温州市农业高新园区开放性项目(KN20210005)
温州市科技特派员专项项目(X20210018)
乐清市农业重点科技项目(2022N026,2022N046)
温州市科协服务科技创新项目(kjfw73)。
关键词
铁皮石斛
糖苷水解酶GH3
生物信息学分析
基因表达模式
Dendrobium officinale
glycoside hydrolase GH3
bioinformatics analysis
gene expression pattern
