摘要
目的探讨温州地区无偿献血人群戊型肝炎病毒感染状况。方法选取温州地区2022年10月至2023年3月成功捐献血液时同步留取的5241例献血者血液标本,同时对戊型肝炎病毒(HEV)进行HEV-IgG抗体、HEV-IgM抗体及核酸(HEV RNA)检测,并对HEV RNA检测为阳性的样本进行定量检测及测序。收集献血者献血登记资料,记录各检测结果阳性率,并分析不同人群HEV感染状况。结果HEV-IgG抗体检测阳性率为23.60%(1237/5241),HEV-IgM抗体检测阳性率为0.29%(15/5241),HEV RNA检测阳性率为0.08%(4/5241)。HEV-IgG检测阳性率随年龄增长而增长(χ^(2)=379.49,P<0.05),男性阳性率高于女性(χ^(2)=5.12,P<0.05)。HEV-IgG及HEV-IgM检测阳性率均随献血次数增加而升高(χ^(2)=40.87、8.30,均P<0.05),2023年阳性率高于2022年(χ^(2)=51.03,13.35,均P<0.05),春季阳性率高于冬季(χ^(2)=51.03、13.35,均P<0.05)。HEV RNA阳性献血者仅有1例HEV-IgG及HEV-IgM抗体检测均呈阳性,其余3例均为阴性,仅有1例测出基因分型,基因分型为Ⅳ型。结论浙江省温州地区无偿献血人群中HEV感染率较高,流行HEV基因分型为Ⅳ型,性别、年龄及献血次数等分布特征可作为辅助筛查参考指标。
Objective To investigate the status of hepatitis E virus infection in unpaid blood donors in Wenzhou.Methods A total of 5241 blood samples were selected from donors who successfully donated blood between October 2022 and March 2023.These samples were tested for hepatitis E virus(HEV)through HEV-IgG antibodies,HEV-IgM antibodies,and nucleic acid tests(HEV RNA).Samples that tested positive for HEV RNA underwent quantitative detection and sequencing.Data regarding the blood donors were collected,the positive rates of each test result were recorded,and the HEV infection status among different populations was analyzed.Results The positive rate of the HEV-IgG antibody test was 23.60%(1237/5241),while the positive rate of the HEV-IgM antibody test was 0.29%(15/5241).The positive rate of the HEV RNA test was 0.08%(4/5241).The positive rate of the HEV-IgG test increased with age(χ^(2)=379.493,P<0.05),and males had a higher positive rate than females(χ^(2)=5.12,P<0.05).Both the positive rates of HEV-IgG and HEV-IgM tests increased as the number of blood donations increased(χ^(2)=40.87,8.30,both P<0.05).Furthermore,the positive rates were significantly higher in 2023 than in 2022(χ^(2)=51.03,13.35,both P<0.05),and they were also significantly higher in spring than in winter(χ^(2)=51.03,13.35,both P<0.05).Only one HEV RNA-positive blood donor tested positive for both HEV-IgG and HEV-IgM antibodies,while the remaining three donors tested negative.Only one case was genotyped,and it belonged to genotypeⅣ.Conclusion The incidence of HEV infection among unpaid blood donors in Wenzhou of Zhejiang Province is relatively high,with the prevalent HEV genotype being typeⅣ.Characteristics such as sex,age,and the number of blood donations can be utilized as auxiliary screening reference indicators.
作者
蔡淑锋
朱紫苗
刘燕飞
徐伟瑞
朱诗瑶
叶璐璐
Cai Shufeng;Zhu Zimiao;Liu Yanfei;Xu Weirui;Zhu Shiyao;Ye Lulu(Department of Laboratory Medicine(Wenzhou Key Laboratory of Blood Transfusion Medicine),Wenzhou Central Blood Station,Wenzhou 325800,Zhejiang Province,China)
出处
《中国基层医药》
CAS
2024年第6期863-868,共6页
Chinese Journal of Primary Medicine and Pharmacy
基金
浙江省温州市基础性公益科研项目(Y20220303)。
关键词
人群监测
戊型肝炎
肝炎
病毒性
人
传染病
肝炎抗体
流行病学研究
Population surveillance
Hepatitis E
Hepatitis,viral,human
Communicable diseases
Hepatitis antibodies
Epidemiologic studies
