摘要
前胡存在早期抽薹的问题,其抽薹机制尚不明确。本研究采用DNBSEQ二代测序技术,分别对抽薹和未抽薹的前胡的茎、叶、根的木质部和韧皮部进行转录组测序,并根据测序结果对前胡进行生物信息学分析。转录组测序分析结果表明,共获得51.21 Gb数据,Trinity组装获得178764条unigenes,再与NR、Swiss-Port、KOG、KEGG、GO等蛋白功能数据库进行比对注释,NR注释到前胡与黄胡萝卜同源序列最多,KOG功能分类分析共获得24个不同的KOG功能类群。注释到GO数据库中共收录818428条unigenes,涉及参与生物过程(BP)、细胞组分(CC)和分子功能(MF)3个过程。KEGG注释到抽薹前胡与未抽薹前胡叶片共有22752个差异基因,在差异基因中筛选与前胡抽薹相关的基因。成花整合因子FT抽薹后较抽薹前表达上调,CCA1、ZTL表达上调,FKF1表达下调。对抽薹前胡与未抽薹前胡差异表达基因的研究,获得抽薹前后前胡转录组信息特征,为后续对前胡抽薹机制的研究,功能基因的挖掘提供科学依据。
The problem of early bolting in Peucedanum praaeruptorum Dunn.,and its bolting mechanism is still unclear.In this study,DNBSEQ second generation sequencing technology was used to conduct transcriptome sequencing on the xylem and phloem of stems,leaves and roots of bolted and unbolted prohu,respectively,and bioinformatics analysis was conducted based on the sequencing results.A total of 51.21 Gb data were obtained by transcriptome sequencing analysis,and 178764 unigenes were assembled by Trinity,which were compared and annotated with NR,Swiss-Port,KOG,KEGG,GO and other protein databases,and the results showed that P.praaeruptorum and Daucus carota subsp.sativus homologous sequences were the most,and 24 different KOG functional taxa were obtained by KOG functional classification analysis.A total of 818428 unigenes are involved in 3 GO categories of biological processes(BP),cellular components(CC),and molecular functions(MF).KEGG annotated that there were a total of 22752 differential genes between the leaves of underwent and did not undergo bolting.The genes related to premature bolting were screened out.The expression of the flowering integration factor FT was up-regulated compared with that before pumping,the expression of CCA1 and ZTL was up-regulated,and the expression of FKF1 was down-regulated.The study of the DEGs between bolting and control group of P.praaeruptorum genes.Obtain the information characteristics of the transcriptome of P.praaeruptorum,and provide scientific basis for the subsequent research on the bolting mechanism of P.praaeruptorum and the mining of functional genes.
作者
杨明霞
韩荣春
张虹
麻泽芊
刘守金
Yang Mingxia;Han Rongchun;Zhang Hong;Ma Zeqian;Liu Shoujin(School of Pharmacy,Anhui University of Chinese Medicine,Hefei,230013;Institute of Traditional Chinese Medicine Resources Protection and Development,Anhui Academy of Chinese Medicine,Hefei,230013)
出处
《分子植物育种》
CAS
北大核心
2024年第14期4566-4573,共8页
Molecular Plant Breeding
基金
道地药材生态种植及质量保障(OP2100000352)资助。
关键词
前胡
转录组
差异表达基因
抽薹
Peucedanum praaeruptorum Dunn.
Transcriptome
Differentially expressed genes
Bolting
