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Peritoneal fluid indocyanine green test for diagnosis of gut leakage in anastomotic leakage rats and colorectal surgery patients

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摘要 BACKGROUND Application of indocyanine green(ICG)fluorescence has led to new developments in gastrointestinal surgery.However,little is known about the use of ICG for the diagnosis of postoperative gut leakage(GL).In addition,there is a lack of rapid and intuitive methods to definitively diagnose postoperative GL.AIM To investigate the effect of ICG in the diagnosis of anastomotic leakage in a surgical rat GL model and evaluate its diagnostic value in colorectal surgery patients.METHODS Sixteen rats were divided into two groups:GL group(n=8)and sham group(n=8).Approximately 0.5 mL of ICG(2.5 mg/mL)was intravenously injected postoperatively.The peritoneal fluid was collected for the fluorescence test at 24 and 48 h.Six patients with rectal cancer who had undergone laparoscopic rectal cancer resection plus enterostomies were injected with 10 mL of ICG(2.5 mg/mL)on postoperative day 1.Their ostomy fluids were collected 24 h after ICG injection to identify the possibility of the ICG excreting from the peripheral veins to the enterostomy stoma.Participants who had undergone colectomy or rectal cancer resection were enrolled in the diagnostic test.The peritoneal fluids from drainage were collected 24 h after ICG injection.The ICG fluorescence test was conducted using OptoMedic endoscopy along with a near-infrared fluorescent imaging system.RESULTS The peritoneal fluids from the GL group showed ICG-dependent green fluorescence in contrast to the sham group.Six samples of ostomy fluids showed green fluorescence,indicating the possibility of ICG excreting from the peripheral veins to the enterostomy stoma in patients.The peritoneal fluid ICG test exhibited a sensitivity of 100%and a specificity of 83.3%for the diagnosis of GL.The positive predictive value was 71.4%,while the negative predictive value was 100%.The likelihood ratios were 6.0 for a positive test result and 0 for a negative result.CONCLUSION The postoperative ICG test in a drainage tube is a valuable and simple technique for the diagnosis of GL.Hence,it should be employed in clinical settings in patients with suspected GL.
机构地区 Department of Surgery
出处 《World Journal of Gastrointestinal Surgery》 SCIE 2024年第6期1825-1834,共10页 世界胃肠外科杂志(英文版)(电子版)
基金 Supported by the Science and Technology Projects in Guangzhou,No.202201010623 and No.2024A03J1016 Guangzhou Science and Technology Project of Traditional Chinese Medicine and Combined Chinese and Western Medicine,No.20242A011001 Guangdong Medical Science and Research Foundation,No.A2024088.
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  • 1[1]Efron EF,Vernava Ⅲ AM.Reoperative surgery for acute colorectal anastomotic dehiscence and persistent abdominal sepsis.In:Longo WE,Northover JMA.Reoperative colon and rectal surgery.London:Martin Dunitz Ltd,2003:1-26
  • 2[2]Golub R,Golub RW,Cantu R Jr,Stein HD.A multivariate analysis of factors contributing to leakage of intestinal anastomoses.J Am Coll Surg 1997; 184:364-372
  • 3[3]McArdle CS,McMillan DC,Hole DJ.Impact of anastomotic leakage on long-term survival of patients undergoing curative resection for colorectal cancer.Br J Surg 2005; 92:1150-1154
  • 4[4]Rullier E,Laurent C,Garrelon JL,Michel P,Saric J,Parneix M.Risk factors for anastomotic leakage after resection of rectal cancer.Br J Surg 1998; 85:355-358
  • 5[5]Sorensen LT,Jorgensen T,Kirkeby LT,Skovdal J,Vennits B,Wille-Jorgensen P.Smoking and alcohol abuse are major risk factors for anastomotic leakage in colorectal surgery.Br J Surg 1999; 86:927-931
  • 6[6]Vignali A,Fazio VW,Lavery IC,Milsom JW,Church JM,Hull TL,Strong SA,Oakley JR.Factors associated with the occurrence of leaks in stapled rectal anastomoses:a review of 1,014 patients.J Am Coll Surg 1997; 185:105-113
  • 7[7]Nesbakken A,Nygaard K,Lunde OC.Outcome and late functional results after anastomotic leakage following mesorectal excision for rectal cancer.Br J Surg 2001; 88:400-404
  • 8[8]Alves A,Panis Y,Trancart D,Regimbeau JM,Pocard M,Valleur P.Factors associated with clinically significant anastomotic leakage after large bowel resection:multivariate analysis of 707 patients.World J Surg 2002; 26:499-502
  • 9[9]Benoist S,Panis Y,Alves A,Valleur P.Impact of obesity on surgical outcomes after colorectal resection.Am J Surg 2000;179:275-281
  • 10[10]Fawcett A,Shembekar M,Church JS,Vashisht R,Springall RG,Nott DM.Smoking,hypertension,and colonic anastomotic healing; a combined clinical and histopathological study.Gut 1996; 38:714-718

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