摘要
花青素类物质作为茶叶的主要生物活性成分,其含量和种类代表着茶叶的食用价值。由于茶叶富含蛋白质、糖类、茶多酚、黄酮类、生物碱、矿物质、维生素、色素等多种成分,采用国内现有相关标准测定茶叶基质中花青素类活性物质时,存在难以同时准确定量多种花青素物质含量的实际问题,因此,文章首先对样品前处理条件和色谱、质谱条件进行优化,然后采用高效液相色谱–串联质谱法测定茶叶中6种花青素类物质的含量。其结果表明,该方法在浓度范围为25~2500μg/L内线性关系良好,相关系数R均大于0.99,回收率的范围为91.95%~106.99%,精密度RSD均小于10%,满足同时准确定量分析的要求。该方法操作简便、灵敏度和准确度高,可为茶叶中花青素含量检测标准方法的制定提供参考。
Anthocyanin,as one of the major bioactive components in tea,represents the edible value.Due to the enrichment of tea in protein,sugar,tea polyphenols,flavonoids,alkaloids,minerals,vitamins,pigments and other components,it is difficult to determine the anthocyanin active substances in tea matrix by relevant domestic standards.Thus,this paper established a high-performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)method for the detection of six anthocyanin compounds in tea sample under the optimization of pretreatment conditions and instrument conditions.The established method showed a good linear relationship within the concentration range of 25 to 2500μg/L,with correlation coefficients(R)greater than 0.99,recovery rates ranging from 91.95%to 106.99%,and precision RSD less than 10%,which meet the requirements for analytical performance.The method exhibited advantages such as easy operation,high sensitivity,and high accuracy.These advantages make it suitable for related analytical detections and providing valuable guidance for the establishment of standard methods for anthocyanin content detection in tea.
作者
黄玉坤
汪国胜
骆丝雨
吉海阿各
胡超琼
冯德建
邹燕
叶善蓉
HUANG Yukun;WANG Guosheng;LUO Siyu;JIHAI Age;HU Chaoqiong;FENG Dejian;ZUO Yan;YE Shanrong(School of Food and Bioengineering,Food Microbiology Key Laboratory of Sichuan Province,Xihua University,Chengdu 610039 China;National Institute of Measurement and Testing Technology,Standard and Testing Technology of Tea Key Laboratory of Sichuan Province,Chengdu 610021 China)
出处
《西华大学学报（自然科学版）》
CAS
2024年第4期123-136,共14页
Journal of Xihua University:Natural Science Edition
基金
四川省重点研发项目(2020YFN0154)
茶叶标准与检测技术四川省重点实验室开放课题(STTT202103)。
