摘要
目的探讨过氧化物氧化还原蛋白(PRDX)1、第10号染色体缺失性磷酸酶-张力蛋白同源物基因(PTEN)水平与自身免疫性肝病患者肝功能、疾病活动性的关系。方法选取2021年1月至2022年12月该院收治的83例自身免疫性肝病患者作为研究对象,根据入院时疾病活动性分为活动期组(37例)、缓解期组(46例),统计两组临床资料及入院时血清PRDX1、PTEN水平,同时对患者进行肝功能Child-Pugh分级并分组。选取同期体检的100例健康志愿者作为对照组。采用多因素Logistic逐步回归分析自身免疫性肝病患者疾病活动性的影响因素,采用受试者工作特征(ROC)曲线及曲线下面积(AUC)分析治疗后血清PRDX1、PTEN水平对自身免疫性肝病患者疾病活动性的评估价值。结果与A级组比较,B级组血清PRDX1、PTEN水平差异无统计学意义(P>0.05),而C级组血清PRDX1水平升高,PTEN水平降低(P<0.05);与B级组相比,C级组血清PRDX1水平升高、PTEN水平降低(P<0.05);与对照组比较,缓解期组血清PRDX1、PTEN水平差异无统计学意义(P>0.05),而活动期组血清PRDX1水平升高、PTEN水平降低(P<0.05);与缓解期组相比,活动期组血清PRDX1水平升高、PTEN水平降低(P<0.05)。血清PRDX1、PTEN判断自身免疫性肝病患者疾病活动性的AUC分别为0.750、0.854,二者联合预测的AUC为0.916。活动期组患者肝区不适、肝硬化占比高于缓解期组(P<0.05);多因素Logistic逐步回归分析显示,肝区不适(OR=3.487,95%CI:1.534~7.927),肝硬化(OR=4.289,95%CI:1.744~10.545),PRDX1≥5.22 ng/mL(OR=5.068,95%CI:1.951~13.164),PTEN≤0.31 pg/mL(OR=5.387,95%CI:2.099~13.829)是影响自身免疫性肝病疾病活动性的危险因素(P<0.05)。结论血清PRDX1水平升高、PTEN水平降低与自身免疫性肝病患者肝功能、疾病活动性密切相关,二者对自身免疫性肝病患者具有一定临床评估价值。
Objective To explore the relationship between the levels of peroxidoredoxin(PRDX)1 and chromosome 10 deletion phosphatase-tensin homologous gene(PTEN)and liver function and disease activity in patients with autoimmune liver disease.Methods A total of 83 patients with autoimmune liver disease admitted to the hospital from January 2021 to December 2022 were selected as the study objects.According to the disease activity at admission,they were divided into active group(37 cases)and remission group(46 cases).Clinical data and serum PRDX1 and PTEN levels of the two groups were analyzed.At the same time,Child-Pugh classification of liver function was performed,and the patients were grouped.A total of 100 healthy volunteers who underwent physical examination during the same period were selected as the control group.Multivariate Logistic regression was used to analyze the influencing factors of disease activity in patients with autoimmune liver disease,and the evaluation value of serum PRDX1 and PTEN levels on disease activity in patients with autoimmune liver disease after treatment was analyzed by receiver operating characteristic(ROC)curve and area under the curve(AUC).Results Compared with the grade A group,there were no significant differences in serum PRDX1 and PTEN levels in the grade B group(P>0.05),while serum PRDX1 level was increased and PTEN level was decreased in the grade C group(P<0.05).Compared with the grade B group,the serum PRDX1 level was increased and PTEN level was decreased in the grade C group(P<0.05).Compared with the control group,there were no significant differences in serum PRDX1 and PTEN levels in the remission group(P>0.05),while the serum PRDX1 level was increased and PTEN level was decreased in the active group(P<0.05).Compared with the remission group,the level of serum PRDX1 was increased and the level of PTEN was decreased in the active group(P<0.05).The AUC of serum PRDX1 and PTEN for evaluating the disease activity in autoimmune liver disease patients was 0.750 and 0.854,respectively,and the AUC of the combined detection of serum PRDX1 and PTEN was 0.916.The proportion of patients with hepatic discomfort and cirrhosis in the active stage group was higher than that in the remission stage group(P<0.05).Multivariate Logistic stepwise regression analysis results showed that hepatic discomfort(OR=3.487,95%CI:1.534-7.927),cirrhosis(OR=4.289,95%CI:1.744-10.545),PRDX1≥5.22 ng/mL(OR=5.068,95%CI:1.951-13.164),PTEN≤0.31 pg/mL(OR=5.387,95%CI:2.099-13.829)were risk factors for disease activity of autoimmune liver disease(P<0.05).Conclusion The increase of serum PRDX1 level and the decrease of serum PTEN level are closely related to liver function and disease activity in patients with autoimmune liver disease,and they have certain clinical evaluation value in patients with autoimmune liver disease.
作者
李青
周路艳
谭智
刘灵芝
LI Qing;ZHOU Luyan;TAN Zhi;LIU Lingzhi(Department of Clinical Laboratory,the First Affiliated Hospital of Hunan College of Traditional Chinese Medicine(Hunan Provincial Hospital of Traditional Chinese Medicine),Zhuzhou,Hunan 412000,China)
出处
《国际检验医学杂志》
CAS
2024年第14期1682-1686,共5页
International Journal of Laboratory Medicine
基金
湖南省科学技术厅项目(S2019JJKWLH0035)。
