摘要
目的探究非小细胞肺癌(NSCLC)患者血清嗜酸性粒细胞趋化因子(CCL11)、脂质转运蛋白-2(LCN-2)水平及其对NSCLC的诊断价值。方法选取2019年10月至2022年12月该院收治的NSCLC患者为NSCLC组(80例),另选取同期在该院进行诊治的80例肺部良性病变患者及80例健康志愿者为良性对照组和健康对照组。采用酶联免疫吸附试验(ELISA)测定血清CCL11、LCN-2水平;比较3组受试者一般资料及血清CCL11、LCN-2水平;采用受试者工作特征(ROC)曲线分析血清CCL11、LCN-2对NSCLC的诊断价值;采用多因素Logistic回归分析NSCLC发生的影响因素。结果NSCLC组血清CCL11、LCN-2水平均显著高于良性对照组和健康对照组(P<0.05),而良性对照组与健康对照组间差异无统计学意义(P>0.05);不同性别、年龄、病理类型NSCLC患者血清CCL11、LCN-2水平比较,差异无统计学意义(P>0.05),有吸烟史、从事高油烟、粉尘等相关工作、中低分化程度、肿瘤最大径>3 cm、淋巴结发生转移、TNM分期为Ⅲ~Ⅳ期的NSCLC患者血清CCL11、LCN-2水平均显著高于无吸烟史、未从事高油烟、粉尘等相关工作、高分化程度、肿瘤最大径≤3 cm、淋巴结未转移、TNM分期为Ⅰ~Ⅱ期的NSCLC患者(P<0.05);ROC曲线结果显示,CCL11、LCN-2单独及二者联合评估诊断NSCLC的曲线下面积分别为0.849、0.841、0.926,灵敏度分别为73.8%、75.0%、85.0%,特异度分别为70.1%、70.0%、75.0%,二者联合诊断NSCLC的效能高于二者单独诊断(P<0.05);多因素Logistic回归分析结果显示,吸烟、从事高油烟、粉尘等相关工作及血清CCL11、LCN-2水平均是NSCLC发生的影响因素(P<0.05)。结论在NSCLC患者中,血清CCL11、LCN-2水平均显著增加,且与NSCLC患者临床病理特征密切相关,二者在诊断NSCLC中具有一定的临床价值。
Objective To investigate the levels of serum CC chemokine ligand-11(CCL11)and lipocalin-2(LCN-2)in patients with non-small cell lung cancer(NSCLC)and their diagnostic value for NSCLC.Methods NSCLC patients admitted to the hospital from October 2019 to December 2022 were collected as the NSCLC group(80 cases),and 80 patients with benign lung lesions and 80 healthy volunteers treated in the hospital during the same period were collected as benign control group and health control group.Enzyme linked immunosorbent assay(ELISA)was applied to measure serum levels of CCL11 and LCN-2.The general data and serum levels of CCL11 and LCN-2 were compared among the three groups.Receiver operating characteristic(ROC)curve was applied to analyze the diagnostic value of serum CCL11 and LCN-2 for NSCLC.Logistic regression was applied to analyze the influencing factors of the occurrence of NSCLC.Results The serum levels of CCL11 and LCN-2 in NSCLC group were obviously higher than those in benign control group and health control group(P<0.05),but there was no statistically significant difference between begin control group and health control group(P>0.05).There was no statistically significant difference in serum CCL11 and LCN-2 levels among NSCLC patients of different genders,ages,and pathological types(P>0.05),the serum levels of CCL11 and LCN-2 in NSCLC patients with a history of smoking,engaged in work related oil smoke at dust,medium to low differentiation,tumor diameter>3 cm,lymph node metastasis,and TNM stagingⅢ-Ⅳwere obviously higher than those in NSCLC patients without a history of smoking,not engaged in work related oil smoke at dust,high differentiation,tumor diameter≤3 cm,non lymph node metastasis,and TNM stagingⅠ-Ⅱ(P<0.05).The results of ROC curve showed that the area under the curve(AUC)of CCL11,LCN-2 alone and their combination in the diagnosis of NSCLC was 0.849,0.841 and 0.926 respectively,the sensitivity was 73.8%,75.0%and 85.0%respectively,and the specificity was 70.1%,70.0%and 75.0%respectively.The efficacy of combined detection of the two in diagnosing NSCLC was better than those of single detection(P<0.05).The results of Logistic regression analysis showed that smoking,engaging in high oil fume,dust related works,and serum levels of CCL11 and LCN-2 were all influencing factors for the occurrence of NSCLC(P<0.05).Conclusion In NSCLC patients,the levels of serum CCL11 and LCN-2 obviously increase,and they are closely related to the clinical pathological characteristics of NSCLC patients.Both have certain clinical value in the diagnosis of NSCLC.
作者
霍霄鸥
张之君
刘慧东
HUO Xiaoou;ZHANG Zhijun;LIU Huidong(Department of Oncology,Taiyuan Central Hospital(Taiyuan Hospital,Peking University First Hospital),Taiyuan,Shanxi 030009,China;Department of Urology,Taiyuan Central Hospital(Taiyuan Hospital,Peking University First Hospital),Taiyuan,Shanxi 030009,China)
出处
《国际检验医学杂志》
CAS
2024年第14期1745-1749,1755,共6页
International Journal of Laboratory Medicine
