摘要
结核病是一种全球范围内的慢性传染病,对人类健康已构成严重威胁,早期诊断、耐药筛查和控制疾病传播是结核病防治的关键方面,然而,现有的诊断技术和药敏试验耗时长,难以实现早期诊断和耐药筛查的目的,大大限制了对疾病传播的控制。数字聚合酶链反应(dPCR)是第3代PCR,是一种灵敏度高、不需要校正曲线的核酸定量检测方法。该文就dPCR的原理及其在结核病诊断、耐药筛查和传播监测中的应用进行综述,并将dPCR与其他结核病检测方法进行比较,展望了dPCR在临床结核病实验室应用中的挑战和未来的前景。
Tuberculosis is a worldwide chronic infectious disease that has become a serious threat to human health.Early diagnosis,drug resistance screening and control of disease transmission are key aspects of TB prevention and treatment.However,existing diagnostic techniques and drug sensitivity tests are time-consuming and difficult to realize the purpose of early diagnosis and drug resistance screening,which greatly limits the control of disease transmission.Digital polymerase chain reaction(dPCR)is the third generation of polymerase chain reaction(PCR),which is a quantitative nucleic acid assay with high sensitivity and no calibration curve.This article provides an overview of the principles of dPCR and its application in tuberculosis diagnosis,drug resistance screening and transmission monitoring,compares dPCR with other tuberculosis detection methods,and looks at the challenges and future prospects of dPCR in clinical tuberculosis laboratory applications.
作者
林依婷
周春妹(综述)
郭玮(审校)
LIN Yiting;ZHOU Chunmei;GUO Wei(Department of Clinical Laboratory,Shanghai Geriatric Medical Center,Shanghai 201100,China;Department of Clinical Laboratory,Zhongshan Hospital,Fudan University,Shanghai 200032,China)
出处
《国际检验医学杂志》
CAS
2024年第14期1756-1760,1766,共6页
International Journal of Laboratory Medicine
基金
国家自然基金面上项目(81972000,82172348)
上海市临床重点专科建设项目(shslczdzk03302)。
关键词
数字聚合酶链反应
结核病
结核分枝杆菌
耐药基因
digital polymerase chain reaction
tuberculosis
Mycobacterium tuberculosis
drug resistance genes
