摘要
目的:基于公共信息学信息数据库分析肺腺癌和癌旁组织中DNA聚合酶εB亚基(POLE2)表达情况,分析POLE2与肺腺癌患者临床病理特征、预后的关系,初步探讨POLE2参与肺腺癌的信号通路。方法:从TCGA数据库下载肺腺癌患者POLE2表达量和相关临床资料,使用R3.6.3软件提取肺腺癌组织和癌旁组织中POLE2表达量数据,比较两组间的表达差异。将POLE2表达量按照中位数值分为POLE2高表达组和POLE2低表达组,分析POLE2表达水平与肺腺癌临床病理特征的关系。利用单因素和多因素COX回归探讨POLE2与肺腺癌预后的关系,使用Kaplan-Meier(KM)法绘制两组生存曲线,比较POLE2高、低表达组生存差异,并采用GEPIA、OncoLnc在线数据库验证,利用基因富集分析(GSEA)方法分析POLE2在肺腺癌中参与的信号通路。结果:肺腺癌组POLE2的表达量明显高于癌旁组,差异有统计学意义(P<0.05)。POLE2在肺腺癌患者中的表达与T分期(P<0.05)和年龄(P<0.05)有关。POLE2(HR=1.212,95%CI 1.019-1.440,P<0.05)是肺腺癌预后的独立影响因素。TCGA数据库KM生存曲线结果表明,POLE2高表达组的存活时间低于低表达组,差异有统计学意义(P<0.05),与GEPIA、OncoLnc结果一致。POLE2在肺腺癌中主要参与E2F蛋白家族、G2M检验点信号通路,与细胞周期调控有关。结论:肺腺癌患者中POLE2高表达,且POLE2表达水平与T分期、年龄有关,POLE2表达情况可以作为预测肺腺癌预后的指标之一,POLE2可能通过影响多种信号通路传导影响肺腺癌进展。
Objective:To analyzed the difference in POLE2 expression between lung adenocarcinoma and adjacent tissues,as well as how POLE2 relates to clinic pathological characteristics and prognosis in lung adenocarcinoma patients,and preliminarily explored the involvement of POLE2 in the signaling pathway of lung adenocarcinoma,which are based on the public informatics information database.Methods:POLE2 expression and related clinical data of lung adenocarcinoma patients were download from TCGA database.The expression data of POLE2 in lung adenocarcinoma tissues and adjacent tissues were extracted by R4.0 software,and the expression difference between the two groups were compared.The expression of POLE2 was divided into high expression group and low expression group according to the median value,and the relationship between POLE2 expression and clinical pathological characteristics of lung adenocarcinoma were analyzed.Univariate and multivariate factor cox regression were used to analyze the relationship between POLE2 and the prognosis of lung adenocarcinoma.Kaplan Meier method was used to draw a survival curve to compare the survival difference between the high and low expression groups of POLE2.The online GEPIA and OncoLnc databases were used for verification,and the signaling pathway involved in POLE2 in lung adenocarcinoma were analyzed by gene enrichment analysis(GSEA).Results:The expression of POLE2 in lung adenocarcinoma group was significantly higher than that in the adjacent group(P<0.05).The expression of POLE2 in lung adenocarcinoma patients was related to T stage(P<0.05)and age(P<0.05).POLE2(HR=1.212,95%CI:1.019-1.440,P<0.05)was an independent prognostic factor of lung adenocarcinoma.The KM survival curve of TCGA database showed that the survival time of POLE2 high expression group was lower than that of low expression group(P<0.05),which was consistent with GEPIA and OncoLnc results.POLE2 was mainly involved in EF2 protein family and G2M checkoint signaling pathway in lung adenocarcinoma,and is related to cell cycle regulation.Conclusions:POLE2 is highly expressed in patients with Lung adenocarcinoma,and the level of POLE2 is related to T stage and age.POLE2 expression can be used as one of the indicators to predict the prognosis of lung adenocarcinoma,and POLE2 may affect the progression of lung adenocarcinoma by affecting the conduction of various signaling pathways.
作者
王源
辛彩霞
杨海英
陈丹
黄娜
Wang Yuan;Xin Caixia;Yang Haiying;Chen Dan;Huang(Na.1.Department of Respiratory and Critical Care Medicine,Renshou County People’s Hospital,Meishan,Sichuan 620500,China;Respiratory Department of Yunchang Hospital in Renshou County,Meishan,Sichuan 620500,China;Department of Respiratory and Critical Care Medicine,First Affiliated Hospital of Chengdu Medical College,Chengdu,Sichuan 610500,China)
出处
《感染、炎症、修复》
2024年第2期130-135,共6页
Infection Inflammation Repair
基金
国家临床重点专科建设培育科室专项科研项目(No.CYFY2018GLPHX02)
四川省医学科研创新课题(No.Q18041)。
