摘要
目的探索川芎嗪对脂多糖(LPS)诱导的急性肺损伤(ALI)小鼠的保护作用,并探寻其可能的作用机制。方法选取50只健康BABL/c小鼠,随机分为对照组、模型组、地塞米松组(2 mg/kg)、川芎嗪低剂量组(20 mg/kg)和川芎嗪高剂量组(40 mg/kg)。对照组和模型组给予生理盐水,地塞米松组(2 mg/kg)、川芎嗪组(20、40 mg/kg)灌胃给予相应药物。给药1 h后,除对照组外,其余各组小鼠气管滴注20μg LPS。测定小鼠肺湿/干质量比,观察肺组织病理学变化,相应试剂盒检测肺组织超氧化物歧化酶(SOD)水平和丙二醛(MDA)含量,ELISA法检测白细胞介素-1β(1L-1β)和肿瘤坏死因子-α(TNF-α)表达,Western blotting检测Txnip、TRX和p-NF-κB p65蛋白表达。结果川芎嗪可显著缓解LPS诱导的ALI小鼠的肺水肿程度(P<0.01);显著上调SOD活力,下调MDA含量(P<0.05);有效降低小鼠肺组织炎性细胞因子表达水平(P<0.05);降低Txnip、pNF-κB p65的表达,上调TRX的表达(P<0.05)。结论川芎嗪可能通过Txnip/TRX/NF-κB信号通路缓解LPS诱导的ALI。
Objective:To study the protective effect of tetramethylpyrazine(TMP)on acute lung injury(ALI)in⁃duced by lipopolysaccharide(LPS)and to explore its possible mechanism.Methods:50 healthy BABL/c mice were randomly divided into control group,ALI group,dexamethasone(2 mg/kg)group,TMP low dose(20 mg/kg)and TMP high dose(40 mg/kg)groups.The mice were administered with saline in control and ALI groups,while dexamethasone and TMP by gavage in other groups accordingly.Except control group,the mice were given intratra⁃cheal instillation of 20μg LPS 1 h after administration.The lung wet/dry weight ratio was measured,the lung his⁃topathological changes were observed,the corresponding kit was used to detect the level of SOD and MDA in lung tissue,the expression of 1L-1βand TNF-αby ELISA,and the expression of Txnip,TRX and p-NF-κB p65 pro⁃tein by Western blotting.Results:TMP significantly reduced the degree of pulmonary edema induced by LPS in ALI mice(P<0.01),increased the level of SOD and decreased the content of MDA(P<0.05),significantly down⁃regulate the levels of inflammatory cytokines in lung tissues of ALI mice(P<0.05).It decreased the protein ex⁃pression of Txnip and p-NF-κB p65,while increased the TRX protein expression(P<0.05).Conclusion:TMP might ameliorate the LPS-induced ALI in mice through Txnip/TRX/NF-κB signaling pathway.
作者
乔勃伟
徐贺
段万石
Qiao Bowei;Xu He;Duan Wanshi(The Second Affiliated Hospital of Air Force Medical University,Shaanxi,Xi′an 710038,China)
出处
《中国中医急症》
2024年第7期1174-1177,共4页
Journal of Emergency in Traditional Chinese Medicine
基金
唐都青年自主创新科学基金项目(2023BTDQN014)。
