摘要
【目的】在黄瓜全基因组中鉴定APETALA2/ethylene responsive factor(AP2/ERF)基因家族,分析其基因结构与功能及表达模式,为该家族基因在黄瓜生长发育及抵御逆境胁迫中的作用提供参考。【方法】利用生物信息学对CsaAP2/ERF家族进行鉴定,并分析该家族成员的理化性质、结构与功能、蛋白互作关系、进化关系和表达模式。【结果】黄瓜全基因组中鉴定出148个AP2/ERF家族成员,根据同源性分为5个亚族:AP2、ERF、DREB、RAV和Soloist,各亚族成员的基因结构与保守基序相似。CsaAP2-11和CsaAP2-15是蛋白互作网络的核心蛋白,与多个蛋白质存在互作关系。共线性分析显示:CsaAP2/ERF与南瓜同源基因进化关系最为相近。在逆境胁迫(冷、热、盐)下,CsaAP2-15、CsaAP2-18、CsaERF-31、CsaERF-54、CsaERF-64、CsaERF-65、CsaDERB-37、CsaDREB-38、CsaDREB-45等基因的表达量发生显著变化。【结论】本研究对黄瓜AP2/ERF家族成员进行系统鉴定和特征分析,并分析了其在多种胁迫下的表达模式,为该家族基因的生物学功能研究与培育抗逆黄瓜新品种提供了理论基础。
[Purpose]To identify the APETALA2/ethylene responsive factor(AP2/ERF) gene family in the genome-wide of cucumber,analyzing its gene structure,function and expression pattern,and providing reference for the role of this family gene in cucumber growth and development and resistance to stress.[Methods]Using bioinformatics to identify the CsaAP2/ERF family,and analyze the physicochemical properties,structure and function,protein interactions,evolutionary relationships,and expression patterns of its members.[Results]148 AP2/ERF family members were identified in the cucumber genome.According to the homology,they were divided into five subfamilies:AP2,ERF,DREB,RAV,and Soloist.The gene structure of each subfamily member was similar to the conservative motif.CsaAP2-11 and CsaAP2-15,as the core proteins of the protein interaction network,interacted with multiple proteins.Collinearity analysis showed that CsaAP2/ERF had the most similar evolutionary relationship with the pumpkin homologous genes.Under stress(cold,heat,salt),the expression levels of genes such as CsaAP2-15,CsaAP2-18,CsaERF-31,CsaERF-54,CsaERF-64,CsaERF-65,CsaDERB-37,CsaDREB-38,and CsaDREB-45 had significantly changed.[Conclusion]In this study,members of the AP2/ERF family in cucumber are systematically identified and characterized,and their expression patterns under various stresses are analyzed,which provide a theoretical basis for the biological function of this family gene and the cultivation of new stress resistant cucumber varieties.
作者
杨巍
赵丽芬
杨娟
李飞
王天文
邓英
YANG Wei;ZHAO Lifen;YANG Juan;LI Fei;WANG Tianwen;DENG Ying(Institute of Horticulture,Guizhou Academy of Agricultural Sciences,Guiyang 550000,China;Guizhou Horticultural Engineering Technology Research Center,Guiyang 550000,China;College of Agriculture,Guizhou University,Guiyang 550025,China;College of Agriculture,Anshun University,Anshun 561000,China)
出处
《云南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2024年第3期127-143,共17页
Journal of Yunnan Agricultural University:Natural Science
基金
2024年贵州省蔬菜产业技术体系(GZSCCYJSTX)
国家大宗蔬菜产业技术体系贵阳综合试验站(CARS-23-G37)
贵州省科技计划项目(黔科合服企[2022]005)
贵州省蔬菜种质资源精准鉴定评价
贵州省农业科学院资源专项(黔农科种质资源[2023]17号)。
关键词
黄瓜
AP2/ERF转录因子
生物信息学
表达模式
Cucumis sativus L.
AP2/ERF transcription factors
bioinformatics
expression pattern
