摘要
为明确东北地区大豆种质资源萌发期耐盐特性,为大豆耐盐性品种选育提供优异亲本,本研究以主要来源于东北地区的348份大豆种质资源为供试材料,在恒温培养箱进行发芽试验,并选用150 mmol·L^(-1)NaCl溶液进行中度盐胁迫处理,测定发芽势、发芽率、芽长和根长,根据发芽率计算相对盐害率,确定耐盐等级并进行耐盐性评价,将筛选出的89份高耐材料使用210 mmol·L^(-1)NaCl溶液进行重度盐胁迫处理,以筛选出萌发期耐盐性稳定材料。结果发现:盐胁迫下各指标测量值与对照相比均明显下降,且各指标之间呈现显著或极显著相关。根据相对盐害率进行耐盐性评价,低盐胁迫下筛选到89份高耐材料,高盐胁迫下筛选到33份耐盐材料。本研究筛选出的33份大豆材料可为耐盐大豆品种培育及大豆耐盐机制解析提供重要基础材料。
In order to clarify the salt tolerance characteristics of soybean germplasm resources in northeast China,and to provide excellent parents for the selection and breeding of salt-tolerant varieties of soybeans,this study took 348 soybean germplasm resources mainly from northeast China as test materials.We cultivated them in an artificial greenhouse,and selected them to be subjected to a low-salt stress treatment with 150 mmol·L^(-1) NaCl during the germination period,and then measured the germination potential,the germination rate,the shoot length and the root length.We calculated the relative salt damage rate and determined the salt tolerance grade and carried out the evaluation of salt tolerance based on the germination rate.The germination potential,shoot length and root length were measured,and the relative salt damage rate was calculated based on the germination rate,and the salt tolerance level was evaluated.89 highly tolerant materials were then subjected to high salt stress with 210 mmol·L^(-1) NaCl solution in order to screen out the materials with stable salt tolerance during the germination period.The results showed that the measured values of all indexes under salt stress decreased significantly compared with the control,and there was a significant or highly significant relationship between the indexes.According to the relative salt damage rate,the salt tolerance was evaluated,89 highly tolerant materials were screened under low salt stress,and 33 salt-tolerant soybean materials were screened under high salt stress.The 33 soybean materials screened in this study can provide important basic materials for breeding salt-tolerant soybean varieties and analyzing the mechanism of salt tolerance in soybean.
作者
李阳阳
董岭超
王英男
赵洪琨
袁翠平
齐广勋
刘晓冬
王玉民
LI Yangyang;DONG Lingchao;WANG Yingnan;ZHAO Hongkun;YUAN Cuiping;QI Guangxun;LIU Xiaodong;WANG Yumin(College of Agriculture,Jilin Agricultural University,Changchun 130118,China;Soybean Research Institute,Jilin Academy of Agricultural Sciences,Changchun 130033,China;Crop Germplasm Resources Institute,Jilin Academy of Sciences,Gongzhuling 136100,China)
出处
《大豆科学》
CAS
CSCD
北大核心
2024年第4期431-438,共8页
Soybean Science
基金
国家重点研发计划(2021YFD1201604-04)
吉林省自然科学基金(20210101007JC)
中国工程院院地合作项目,吉林省种质资源保护与发展战略研究(JL2021-07)。
关键词
大豆
东北地区
萌发期
NACL胁迫
耐盐性鉴定
soybean
northeast China
germination stage
NaCl stress
salt tolerance identfication
