摘要
目的:探讨根皮素调节CC趋化因子配体2(CCL2)-CC趋化因子受体2(CCR2)信号轴对肺癌细胞恶性进展的影响。方法:将A549细胞分为对照组(未进行任何处理的A549细胞)、低剂量根皮素组(500μmoL/L根皮素处理A549细胞)、中剂量根皮素组(750μmoL/L根皮素处理A549细胞)、高剂量根皮素组(1000μmoL/L根皮素处理A549细胞)、高剂量根皮素+GW0742组(1000μmoL/L根皮素与1.0μmoL/L CCL2-CCR2信号通路激活剂GW0742处理A549细胞)。CCK-8法和流式细胞术分别检测A549细胞增殖及凋亡;Transwell法检测细胞迁移和侵袭;Western blot测定CCL2-CCR2信号轴相关蛋白表达。结果:低、中、高剂量根皮素组细胞OD450值、迁移及侵袭数量、CCL2、CCR2蛋白表达均低于对照组(P<0.05),细胞凋亡率高于对照组(P<0.05),且呈剂量依赖性。高剂量根皮素+GW0742组细胞OD450值、迁移及侵袭数量、CCL2、CCR2蛋白表达均高于高剂量根皮素组(P<0.05),细胞凋亡率低于对照组(P<0.05)。结论:根皮素可能通过调节CCL2-CCR2信号轴介导的免疫反应进而抑制肺癌细胞的恶性进展。
Objective:To investigate the effect of phloretin on the CC chemokine ligand 2(CCL2)-CC chemokine receptor 2(CCR2)signaling axis on the malignant progression of lung cancer cells.Methods:A549 cells were grouped into a control group(untreated A549 cells),a low-dose phloretin group(500μmoL/L phloretin treated A549 cells),a medium-dose phloretin group(750μmoL/L phloretin treated A549 cells),a high-dose phloretin group(1000μmoL/L phloretin treated A549 cells),and a high-dose phloretin+GW0742 group(1000μmoL/L phloretin and 1.0μmoL/L CCL2-CCR2 signaling pathway activator GW0742 co-treated A549 cells).CCK-8 method and flow cytometry were applied to detect A549 cell proliferation and apoptosis,respectively;the Transwell method was applied to detect cell migration and invasion;Western blot was applied to determine the expression of CCL2-CCR2 signaling axis-related proteins.Results:The OD450 value,migration and invasion numbers,and the CCL2,CCR2 protein expression in the low,medium,and high dose phloretin groups were lower than those in the control group(P<0.05),the apoptosis rate of cells was higher(P<0.05),in a dose-dependent manner.The OD450 value,migration and invasion numbers,and the CCL2,CCR2 protein expression in the high dose phloretin+GW0742 group were higher than those in the control group(P<0.05),the apoptosis rate of cells was lower than that in the control group(P<0.05).Conclusion:Phloretin may inhibit the malignant progression of lung cancer cells by regulating the CCL2-CCR2 signaling axis-mediated immune response.
作者
蒋莎莎
杨鹏
荣丽雯
曹航
JIANG Shasha;YANG Peng;RONG Liwen(Chengdu Third People's Hospital/Affiliated Hospital of Southwest Jiaotong University,Sichuan Chengdu 610031,China)
出处
《河北医学》
CAS
2024年第7期1057-1061,共5页
Hebei Medicine
基金
四川省成都市科技项目,(编号:2022-YF05-02119-SN)。
