摘要
本文以聚丙烯酸(PAA)、正硅酸乙酯(TEOS)、硅烷偶联剂Si-69为主要原料,异硫氰酸荧光素(FITC)为荧光剂,利用自模板法制备具有荧光标记的中空介孔SiO_(2)纳米载体(HMSNs-69-FITC)。通过FTIR、DLS、BET、Raman和TEM对纳米载体的结构、粒径进行测定,用紫外分光光度计和TEM对其还原敏感性能进行表征,用溶剂挥发法负载索拉非尼(SOR),并计算其负载效率。研究结果表明:调控PAA的量可以实现HMSNs在28~380 nm范围内粒径可控,其中,0.024 g/mL PAA、平均粒径100 nm的HMSNs稳定性能优良,HMSNS-69-FITC对SOR的负载效率为280.0μg/mg,在含有0.0083 g/mL二硫苏糖醇(DTT)的磷酸缓冲盐溶液(PBS)溶液中,48 h累计释放率约为82.4%;而无DTT时,48 h累计释放率约为25.1%,该载体具有二硫键还原敏感性。此工作有助于推动粒径可控的、还原敏感型SiO2纳米载体领域的研究。
In this paper,hollow mesoporous SiO_(2) nanocarriers(HMSNs-69-FITC)with fluorescence labeling were prepared by self-template method using polyacrylic acid(PAA),tetraethyl orthosilicate(TEOS)and silane coupling agent Si-69 as the main raw materials and fluorescein isothiocyanate(FITC)as the fluorescent agent.The structure and particle size of nanocarriers were determined by FTIR,DLS,BET,Raman and TEM,their reduction-sensitive properties were characterized by ultraviolet spectrophotometer and TEM,and sorafenib(SOR)was loaded with solvent volatilization,and calculate its load efficiency.The results show that the amount of regulating PAA can achieve controllable particle size of HMSNs in the range of 28-380 nm,among which,HMSNs with 0.024 g/mL PAA and an average particle size of 100 nm have excellent stability performance,HMSNs-69-FITC has a loading efficiency of 280.0μg/mg for SOR,and in phosphate buffered saline(PBS)solution containing 0.0083 g/mL dithiothreitol(DTT),the cumulative release rate of 48 h is about 82.4%.In the absence of DTT,the cumulative release rate at 48 h is about 25.1%,which has significant disulfide bond reduction sensitivity.This work helps advance research in the field of particle size controllable and reduction-sensitive SiO_(2) nanocarriers.
作者
尹城武
陈雨鑫
王钰杰
朱德会
尹付琳
成琳
赵宇
周国永
YIN Chengwu;CHEN Yuxin;WANG Yujie;ZHU Dehui;YIN Fulin;CHENG Lin;ZHAO Yu;ZHOU Guoyong(School of Chemical Engineering,Guizhou Minzu University,Guiyang 550025,China;College of Ethnic Medicine,Guizhou Minzu University,Guiyang 550025,China)
出处
《复合材料学报》
EI
CAS
CSCD
北大核心
2024年第6期3182-3192,共11页
Acta Materiae Compositae Sinica
基金
贵州省省级科技计划项目(黔科合基础-ZK[2022]一般216)
贵州省高等学校绿色化学与资源环境创新团队(黔科技[2022]13号)
贵州民族大学科研项目(GZMUZK[2021]YB09)。
关键词
二硫键还原敏感型
纳米药物载体材料
粒径可控
中空介孔SiO_(2)
聚丙烯酸
功能化修饰
disulfide bond reduction sensitive type
nanodrug carrier materials
controlled particle size
hollow mesoporous SiO_(2)
polyacrylic acid
functional modification
