摘要
本文旨在研究天然冰片(natural borneol,NB)对宫颈癌细胞增殖、凋亡及细胞迁移的影响及其作用机制。运用倒置显微镜观察记录细胞的表型变化,发现NB可显著改变SiHa与HeLa细胞的形态,使细胞变圆,胞间连接变松散;采用CCK-8法和集落形成实验发现NB处理SiHa与HeLa细胞明显抑制细胞增殖;流式细胞检测结果表明,NB处理组能显著诱导细胞凋亡,且呈现剂量依赖性;Transwell实验和划痕实验表明,NB能抑制SiHa与HeLa细胞的侵袭与迁移能力;蛋白免疫印迹结果表明,NB处理后下调Bcl-2、缺氧诱导因子-1α(HIF-1α)、Erk1/2、Snail与N-cadherin蛋白表达,同时上调p-Erk1/2蛋白表达;mRNA测序结合信号通路富集结果显示NB的作用机制涉及MAPK、细胞凋亡等相关的信号通路。综上,NB可能通过影响MAPK、HIF-1α信号通路抑制宫颈癌细胞增殖和迁移,诱导细胞凋亡。
The purpose of this study was to investigate the effect and the mechanism of natural borneol(NB)on the proliferation,apoptosis and migration of cervical cancer cells.The phenotypic changes of cells were observed and recorded by inverted microscope,and found NB could significantly alter the morphology of SiHa and HeLa cells,which causing the cells turn to round and loose the intercellular connection.CCK-8 assay and colony formation assay showed that NB significantly inhibited SiHa and HeLa cells proliferation and colony formation.Flow cytometry results showed that NB induced cell apoptosis in a dose-dependent manner.Transwell and scratch assay showed that NB could inhibit the invasion and migration of SiHa and HeLa cells.Western blot results demonstrated that NB downregulated the expression of Bcl-2,hypoxia-inducible factor-1α(HIF-1α),Erk1/2,Snail as well as N-cadherin,and upregulated the expression of p-Erk1/2.mRNA sequencing combined with signal pathway enrichment results showed that the mechanism of NB mainly involved in MAPK signaling pathway and cell apoptosis.In conclusion,NB may inhibit the proliferation and migration of cervical cancer cells,induce cell apoptosis through MAPK and HIF-1αsignaling pathway.
作者
李振豪
黎灵
刘婉榆
王楠
LI Zhen-hao;LI Ling;LIU Wan-yu;WANG Nan(School of Life Science,Jiaying University;Guangdong Provincial Key Laboratory of Conservation and Precision Utilization of Characteristic Agricultural Resources in Mountainous Areas,Meizhou 514015,China)
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2024年第7期1191-1199,共9页
Natural Product Research and Development
基金
广东省科技创新战略专项(20230104)
广东省高校科研项目(021KTSCX126)
广东大学生科技创新培育专项(pdjh2020a0547)。
关键词
天然冰片
宫颈癌
细胞增殖
细胞凋亡
细胞迁移
natural borneol
cervical cancer
cell proliferation
apoptosis
cell migration
