摘要
实验旨在研究饲料中添加辅酶Q10对大口黑鲈生长、体组成、抗氧化能力,以及在低氧应激下血清、肝脏抗氧化酶活性的影响,实验设计3种等氮等脂饲料,分别为400 g/kg鱼粉对照组(F0)、200 g/kg鱼粉对照组(F1)、200 g/kg鱼粉+50 mg/kg辅酶Q10(F2),以初始体重为(5.00±0.02)g的大口黑鲈为研究对象,进行为期56 d的养殖实验,实验结束1周后进行3~4 mg/L的低氧应激,并取样分析。结果表明:相比F1对照组,饲料中添加50 mg/kg辅酶Q10的增重率(WG)、特定生长率(SGR)和末均重(FW)显著提高(P<0.05);体成分中添加50 mg/kg辅酶Q10的粗蛋白含量最高,全鱼水分和粗脂肪含量各组无显著差异(P>0.05);添加辅酶Q10血清谷草转氨酶(AST)的活性显著降低(P<0.05),其中50 mg/kg辅酶Q10组血清谷胱甘肽过氧化物(GSH-Px)、谷胱甘肽(GSH)和过氧化氢酶(CAT)活性均有提高(P>0.05)。饲料中添加50 mg/kg辅酶Q10的血清丙二醛(MDA)含量显著低于高鱼粉对照F0组(P<0.05)。在低氧应激条件下,添加辅酶Q10的血清GSH-Px和CAT活性相比F0组显著提高(P<0.05),而肝脏中相比其他实验组添加50 mg/kg辅酶Q10的GSH-Px和CAT活性最高。皮质醇含量50 mg/kg辅酶Q10相比高鱼粉对照F0组显著下降(P<0.05)。综上所述,饲料中添加50 mg/kg辅酶Q10能够促进大口黑鲈的生长性能,提高机体的抗氧化能力和低氧应激下的抗胁迫能力。
The aim was to investigate the effect of feed supplementation with coenzyme Q10 on Microp-terus Salmoides growth,body composition,antioxidant capacity,and serum and liver antioxidant enzyme activity under low oxygen stress.Three isonitrogenous and isoenergetic diets were formulated,400g/kg fish meal control(F0),200 g/kg fish meal control(F1),200 g/kg fish meal+50 mg/kg coenzyme Q10(F2).The diets were fed to M.salmoides with the initial body weight of(5.00±0.02)g for 56 daysHypoxic stress at 3-4 mg/L was performed 1 week after the end of the experiment and sampled for analysis.The results showed as follows:Compared with the control group F1,WG,SGR and FW supplemented with 50mg/kg coenzyme Q10 were significantly increased(P<0.05).The highest crude protein was 50 mg/kg coenzyme Q10.Fish water and crude fat content were not significantly different between groups(P>0.05).The serum of AST supplemented with coenzyme Q10 was significantly decreased(P<0.05).The serum GSH-Px,GSH and CAT activities in the 50 mg/kg coenzyme Q10 group were higher than the control group F1(P>0.05),and the serum MDA content was significantly lower than the high fish meal control group F0(P<0.05).Under low-oxygen stress conditions,significantly increased serum GSH-Px and CAT activity supplemented with co-enzyme Q10(P<0.05),GSH-Px and CAT activity with 50 mg/kg coenzyme Q10 were the highest in liver compared with other experimental groups.The cortisol content of 50 mg/kg Coenzyme Q10 was signifi-cantly lower than that of F0 group(P<0.05).In conclusion,the addition of 50 mg/kg Coenzyme Q10 to the feed can promote the growth performance of M.salmoides,improve the antioxidant capacity of the body and the anti stress capacity under hypoxia stress.
作者
舒斌
李建东
张磊
吕小燕
陈颖
吴坤岚
龚洋洋
SHU Bin;LI Jiandong;ZHANG Lei;LYU Xiaoyan;CHEN Ying;WU Kunlan;GONG Yangyang(Zhejiang NHU Co.,Ltd.,Zhejiang Shaoxing 312500,China)
出处
《饲料工业》
CAS
北大核心
2024年第14期56-62,共7页
Feed Industry
关键词
辅酶Q10
生长
体组成
抗氧化能力
低氧胁迫
大口黑鲈
coenzyme Q10
growth
body composition
antioxidant ability
hypoxia stress
Micropterus salmoides
