摘要
目的探讨制何首乌对脑卒中后抑郁(PSD)大鼠的作用及其机制。方法采用线栓法建立脑中动脉阻塞模型(MCAO),将大鼠随机分为模型组、氟西汀组和制何首乌低、高剂量组,每组10只,另设假手术组。于MCAO造模7d后对除假手术组外的其他大鼠进行持续21d的PSD造模,于第1、7、14、21天进行神经功能及行为学评分,第21天,检测脑梗死面积、脑含水量,采用HE染色、尼氏染色及免疫组化染色观察脑缺血半暗带区病理学形态,ELISA及Westernblot法检测脑组织水通道蛋白(AQP3、AQP4、AQP5)及脑源性神经营养因子(BDNF)蛋白表达。结果与模型组比较,制何首乌高剂量组可改善神经功能及行为学评分(P<0.05,P<0.01),降低脑梗死面积和脑含水量(P<0.05)。与模型组比较,各给药组缺血半暗带区脑部结构清晰,细胞间隙变小,神经元数量增多,脑组织AQP3、AQP4、AQP5蛋白表达降低(P<0.05,P<0.01),而BDNF蛋白表达升高(P<0.05)。结论制何首乌可通过降低AQP3、AQP4、AQP5蛋白表达,消除脑水肿,提高BDNF蛋白表达,改善神经元微环境,促进神经元的生长存活,增强神经功能,从而减弱PSD大鼠的抑郁程度。
AIM To explore the effects and mechanism of Polygoni multiflori Radix Preparata(PMRP)on a rat model of post-stroke depression(PSD).METHODS The models of middle cerebral artery occlusion(MCAO)established by thread embolism method were then randomly divided into the model group,the positive drug(fluoxetine)group,and the low-dose and high-dose PMRP groups,with 10 rats in each group,in contrast to the 10 rats of the sham operation group.After 7 days of MCAO modeling,the rats underwent their 21-day PSD modeling except those of the sham operation group,during which the rats had their the neurological functions and behaviors assessed on the 1st,7th,14th and 21st day;and their cerebral infarction area and brain water content detected on the 21st day.HE staining,Nissl staining and immunohistochemical staining were used to observe the pathological morphology of cerebral ischemic penumbra.ELISA and Western blot were applied in the detections of the cerebral protein expressions of aquaporins(AQP3,AQP4,AQP5)and brain-derived neurotrophic factor(BDNF).RESULTS Compared with the model group,the high-dose PMRP group displayed improvedneurological functions and behavioral scores(P<0.05,P<0.01),and reduced cerebral infarction area and brain water content(P<0.05).Compared with the model group,each treatment group demonstrated clearer brain structure in ischemic penumbra,smaller intercellular space,increased neuron counts,decreased cerebral protein expressions of AQP3,AQP4 and AQP5(P<0.05,P<0.01),and increased BDNF protein expression(P<0.05).CONCLUSION PMRP can reduce the depression of PSD rats by improving the microenvironment of neurons to promote their growth and survival,and eliminating the brain edema to enhance neurological functions via reduced protein expressions of AQP3,AQP4 and AQP5,and increased BDNF protein expression.
作者
刘洋
田洋
冷沁洁
彭静
吴晓青
谭睿
LIU Yang;TIAN Yang;LENG Qin-jie;PENG Jing;WU Xiao-qing;TAN Rui(College of Life Sciences and Engineering,Southwest Jiaotong University,Chengdu 610031,China)
出处
《中成药》
CAS
CSCD
北大核心
2024年第7期2211-2219,共9页
Chinese Traditional Patent Medicine
基金
四川省中医药产业发展重大项目(510201202109711)
四川省中医药管理局团队项目(2022C010)
成都市科技项目“技术创新研发项目”(2021-YF05-00447-SN)。
关键词
制何首乌
脑卒中后抑郁
水通道蛋白
脑源性神经营养因子
Polygoni multiflori Radix Preparata
post-stroke depression
aquaporins
brain-derived neurotrophic factor
