摘要
【目的】挖掘青稞代谢啶磺草胺可能涉及的重要基因,明确青稞对啶磺草胺的代谢解毒机制,为啶磺草胺的科学使用和耐受除草剂的青稞品种选育提供理论基础。【方法】以啶磺草胺处理0、1、6 d的敏感青稞品种青0160以及耐药青稞品种青0306的叶片为材料,借助高通量测序技术进行转录组测序结果分析。【结果】GO富集分析结果表明,啶磺草胺处理前后差异表达基因均显著富集在光合作用上。KEGG富集分析表明,啶磺草胺处理1 d后,谷胱甘肽代谢途径中抗氧化剂相关基因以及苯丙素和吡啶生物碱生物合成途径中与应激相关基因显著富集;啶磺草胺处理6 d后,与维持细胞功能的一系列生物过程及氨基酸的生物合成和代谢相关的基因显著富集。进一步分析表明,两个品种中的SOD21972、POD55052、CAT15170、DHAR59510、APX42784、GR34873、GSTs849、GSTs36979、GSTs31507、GSTs15106、GSTs20762表达上调,PSⅡ55705、Cc31194、Cc17547、Cc17551、CYP12424受到抑制;其中POD55052在敏感青稞中表达量高于耐药青稞,SOD21972、CAT15170、DHAR59510、APX42784、GR34873、GSTs849、GSTs36979、GSTs31507、GSTs15106、GSTs20762、PSⅡ55705、Cc31194、Cc17547、Cc17551、CYP12424在耐药青稞中表达量高于敏感青稞,这些基因可能参与了青稞耐啶磺草胺机制。qRT-PCR相对表达量和转录组测序结果变化趋势基本一致,证明转录组测序结果具有可靠性。【结论】明确了青稞对啶磺草胺的代谢解毒机制,为青稞耐受啶磺草胺提供分子基础,对啶磺草胺安全用药及选育青稞耐除草剂品种有重要意义。
【Objective】Key genes involved in the detoxification of pyroxsulam in naked barley were identified to facilitate breeding an herbicide-tolerant variety.【Method】Leaves of herbicide-sensitive Qing 0160 and herbicide-resistant Qing 0306 naked barley were treated with pyroxsulam for 0,1,or 6 d prior to a high throughput transcriptome sequencing.【Result】The GO of the differentially expressed genes were significantly enriched in photosynthesis before as well as after the pyroxsulam treatment.Significant KEGG enrichment of the genes related to antioxidants in the glutathione metabolic pathway and the stress-related genes in the phenylpropanol and pyridine alkaloid biosynthesis pathways was observed 1d after the treatment.And 6 d afterward,the genes related to the biological processes that maintain the cell function as well as the biosynthesis and metabolism of amino acids were significantly enriched.Further analysis indicated that the expressions of SOD21972,POD55052,CAT15170,DHAR59510,APX42784,GR34873,GSTs849,GSTs36979,GSTs31507,GSTs15106,and GSTs20762 were upregulated,while PSⅡ55705,Cc31194,Cc17547,Cc17551,and CYP12424 downregulated.POD55052 in the herbicide-sensitive naked barley was highly expressed but not in the herbicide-resistant counterparts.On the other hand,the expressions of SOD21972,CAT15170,DHAR59510,APX42784,GR34873,GSTs849,GSTs36979,GSTs31507,GSTs15106,GSTs20762,PSⅡ55705,Cc31194,Cc17547,Cc17551,and CYP12424 were higher in the herbicide-resistant than in the herbicide-sensitive naked barley.It suggested that they might be involved in the pyroxsulam-resistance of the plants.【Conclusion】The basically same trend shown in this study on the gene express by qRT-PCR and the transcriptome sequencing unveiled the genes possibly associated with the pyroxsulam detoxification mechanism of naked barley.The information would facilitate the breeding of an herbicide-resistant variety.
作者
蔡青
翁华
CAI Qing;WENG Hua(Institute of agriculture and animal husbandry,Qinghai University,Xining,Qinghai 810016,China;Academy of Agricultural and Forestry Sciences,Qinghai University,Xining,Qinghai 810016,China)
出处
《福建农业学报》
CAS
CSCD
北大核心
2024年第4期492-502,共11页
Fujian Journal of Agricultural Sciences
基金
青海省科技厅基础研究项目(2023-ZJ-930M)。
关键词
青稞
啶磺草胺
转录组
耐药基因
naked barley
pyroxsulam
transcriptome
drug resistance gene
