摘要
黑龙江省大豆田反枝苋Amaranthus retroflexus对ALS、PPO抑制剂类除草剂产生了严重抗性,为明确化学防除失败的反枝苋对咪唑乙烟酸、氟磺胺草醚的抗性机理,采用整株生物测定法测定了反枝苋抗性及敏感种群对除草剂的敏感性,克隆了抗性和敏感种群的ALS、PPO基因(质体型PPX1及线粒体型PPX2),查找突变位点,将突变基因转入拟南芥Arabidopsis thaliana验证其功能,明确抗性机理。结果显示:反枝苋抗性种群对咪唑乙烟酸、氟磺胺草醚抗性倍数分别为55.29倍和17.51倍,抗性植株ALS存在Ala205Val或Ser653Asn突变,PPX2存在Arg128Gly突变。将抗性种群的PPX2转入拟南芥,可导致拟南芥对氟磺胺草醚产生高水平抗性(10.3倍)。综上,东北大豆田反枝苋对咪唑乙烟酸、氟磺胺草醚产生了高水平多抗性,ALS、PPO基因突变是导致此类抗性的主要原因之一。
In Heilongjiang province,Amaranthus retroflexus has developed resistance to ALS(acetolactate synthase)and PPO(protoporphyrinogen oxidase)inhibitor herbicides in soybean fields.To understand the resistance mechanism of A.retroflexus to imazethapyr and fomesafen,a whole plant bioassay was conducted to determine the susceptibility of resistant and susceptible populations.The ALS and PPO genes(plastid PPX1 and mitochondrial PPX2)from the populations were cloned,and sequence alignment was conducted to find mutations.In addition,the mutated gene was transformed into Arabidopsis thaliana to verify its function.The results showed that the resistance factor for A.retroflexus resistant population to imazethapyr and fomesafen was 55.29 fold and 17.51 fold,respectively.The resistant plants contained the mutations Ala205Val or Ser653Asn in the ALS,and Arg128Gly in the PPX2.The A.thaliana containing mutated PPX2 gene showed high resistance to fomesafen(10.3 fold).In summary,mutations in ALS and PPO genes associate with multiple resistance of A.retroflexus to imazethapyr and fomesafen in soybean fields in northeast China.
作者
杜龙
李宁
尚素琴
李丹
李晓
曲明静
DU Long;LI Ning;SHANG Suqin;LI Dan;LI Xiao;QU Mingjing(National Engineering Technology Research Center of Peanut,Shandong Peanut Research Institute,Qingdao 266100,China;Huanggang Academy of Agricultural Sciences,Huanggang 438000,China;College of Plant Protection,Gansu Agricultural University,Lanzhou 730070,China)
出处
《植物保护》
CAS
CSCD
北大核心
2024年第4期32-38,共7页
Plant Protection
基金
山东省自然科学基金(ZR2020QC135)
国家自然科学基金(32001940)
财政部和农业农村部:国家花生产业技术体系建设专项(CARS-13)。
关键词
反枝苋
氟磺胺草醚
咪唑乙烟酸
多抗性机理
Amaranthus retroflexus
fomesafen
imazethapyr
multiple resistance mechanism
