摘要
目的 探索HPRT1基因在肺腺癌中的表达特征、总生存率、功能激活以及免疫浸润中的影响。方法 通过对TCGA肺腺癌数据以及多个GEO数据库中的肺腺癌数据进行挖掘分析,对比并验证HPRT1表达量与预后总生存(overall surival,OS)的关系,通过cluster Profiler分析HPRT1基因高表达组中上调基因的功能富集情况。且通过TIMER以及CIBERSORT算法计算肺腺癌中不同免疫细胞的浸润水平并对比在HPRT1高低表达组间的浸润程度差异。结果 HPRT1在肺腺癌组织中表达量显著上调,且在TCGA中证明HPRT1基因高表达的患者预后OS更差(P <0.01)。经过2个GEO数据集的验证同样发现HPRT1基因高表达表现为预后OS更差(GSE13213,P <0.01;GSE67639,P <0.001)。差异分析显示高表达的患者中有683个基因的表达量显著上调且这些上调基因的功能主要富集与p53以及细胞周期等癌症相关的信号通路。TIMER以及CIBERSORT算法进行的免疫细胞浸润程度分析发现在高表达差预后人群中B细胞以及CD4T细胞含量均更低(P <0.05)。结论 HPRT1基因表达量越高肺腺癌患者的总生存越差,且高表达患者的p53信号通路上调,B细胞以及CD4T细胞浸润程度显著下降。
Objective To explore the expression characteristics,prognostic effect,functional activation and immune infiltration of HPRT1 gene in lung adenocarcinoma.Methods The data of adenocarcinoma of the lung from TCGA and multiple GEO databases were collected and analyzed.The relationship between HPRT1 expression level and overall survival(OS)was compared and validated,and the functional enrichment of upregulated genes in the HPRT1 high-expression group was analyzed using ClusterProfiler.Furthermore,the infiltration levels of immune cells in lung adenocarcinoma were calculated using TIMER and CIBERSORT algorithms and compared between the HPRT1 high-and low-expression groups.Results The expression of HPRT1 was significantly upregulated in lung adenocarcinoma tissues,and TCGA dataset analysis showed the patients with high expression of HPRT1 gene had a poorer overall survival(OS)prognosis(P<0.01).Validation through two GEO datasets also shows that high expression of the HPRT1 gene is associated with a worse OS prognosis(GSE13213,P<0.01,GSE67639,P<0.001).Differential analysis indicates that among patients with high expression,the expression levels of 683 genes are significantly upregulated,with these upregulated genes primarily enriched in signaling pathways related to cancer,such as p53 and the cell cycle.Analysis of immune cell infiltration levels using TIMER and CIBERSORT algorithms reveals that in the group with high expression and poor prognosis,the levels of B cells and CD4 T cells are lower(P<0.05).Conclusion The higher the expression level of the HPRT1 gene,the significantly worse the overall survival of patients with lung adenocarcinoma,and in patients with high expression levels,the p53 signaling pathway is upregulated,and the infiltration level of B cells and CD4T cells is significantly decreased.
作者
杨红秀
朱中山
YANG Hongxiu;ZHU Zhongshan(Dept.of Oncology,The 2nd People’s Hospital of Hunan Province,Changsha Hunan 410007,China)
出处
《昆明医科大学学报》
CAS
2024年第8期17-23,共7页
Journal of Kunming Medical University
基金
湖南省自然科学基金科卫联合基金资助项目(2020JJ8080)。
