摘要
为了解^(60)Co-γ射线辐照处理对蝴蝶兰生长的影响及辐射处理后植株的变异表现,对‘郑农火凤凰’和‘郑农鸿运’两个蝴蝶兰品种进行不同剂量的^(60)Co-γ辐射处理,探究辐射处理对蝴蝶兰生长的影响,确定合适的诱变剂量范围。结果表明:(1)^(60)Co-γ辐射处理影响蝴蝶兰植株的生长发育,不同品种的蝴蝶兰对辐射的敏感度不同,‘郑农鸿运’对^(60)Co-γ射线更敏感;(2)植株SPAD值随着辐射剂量的增加呈先增加后降低的趋势,‘郑农火凤凰’和‘郑农鸿运’分别在60Gy和40Gy时具有最高SPAD平均值。(3)辐射处理诱变的植株表观变异具有多样性,不同辐射剂量处理均会导致植株叶片增厚。10Gy剂量的辐射处理时植株出现变异,25Gy时花朵出现变异,40Gy及以上剂量的辐射处理抑制蝴蝶兰植株开花,推测蝴蝶兰合适的诱变剂量范围为10~40Gy。研究结果为蝴蝶兰诱变育种提供了理论依据和剂量参考。
To investigate the effect of^(60)Co-γ irradiation on the growth and development of Phalaenopsis orchid and the variation of plants after radiation treatment.Two Phalaenopsis cultivars‘Zhengnong Huofenghuang’and‘Zhengnong Hongyun’were treated with different doses of^(60)Co-γradiation to explore its effect on the growth dynamics and ascertain the optimal range of mutagenesis dose.The results showed that:(1)^(60)Co-γ radiation significantly influenced the growth and development of Phalaenopsis plants,with'Zhengnong Hongyun'exhibiting heightened sensitivity to the irradiation;(2)SPAD values of plants increased first and then decreased with the increase of radiation dose,‘Zhengnong Huofenghuang’and‘Zhengnong Hongyun’had the highest average SPAD values at 60Gy and 40Gy,respectively.(3)Radiation-induced phenotypic variations in plants were diverse,including leaf thickening observed at lower doses(10Gy)and floral alterations at 25Gy.Radiation doses of 40Gy inhibit flowering in Phalaenopsis plants,suggesting an optimal mutagenic dose range of 10~40Gy for Phalaenopsis mutagenesis breeding.This study provides a theoretical foundation and practical dose guidelines for Phalaenopsis mutagenesis breeding in the future.
作者
蒋拴丽
王瑞华
杨书才
冯建
赵玉安
王世尧
王俊
JIANG Shuani;WANG Ruihua;YANG Shucai;FENG Jian;ZHAO YuanWANG Shiyao;WANG Jun(Zhengzhou Institute of Agricultural Science and Technology,Zhengzhou,Henan 450005,China)
出处
《陕西农业科学》
2024年第7期12-18,共7页
Shaanxi Journal of Agricultural Sciences
基金
河南省科技攻关项目-蝴蝶兰及近缘属种质资源收集、评价与新品种选育(232102111101)。
关键词
蝴蝶兰
^(60)Co-γ射线
辐射诱变
叶绿素
变异
Phalaenopsis aphrodite
^(60)Co-γ ray
Radiation mutagenesis
Chlorophyll
Phenotypic variation
