摘要
目的研究厚朴温中汤合煎与单煎药性的一致性,探讨配方颗粒替用传统汤剂的适用性。方法采用透析与柱层析等方法制得厚朴温中汤合煎与单煎总提物及其总多糖、总皂苷、总黄酮、总木脂素和总生物碱。采用CCK-8法研究厚朴温中汤合煎与单煎总提物及其各组分对人胃癌HCG-27细胞体外增殖的影响来评价药性,通过显微镜观察药物对细胞形态的影响,台盼蓝染色观察药物对细胞的毒性。结果合煎与单煎总提物和总多糖在(5~800)μg·mL^(-1) 浓度范围内对HCG-27细胞的增殖影响都表现为促进,无细胞毒作用。合煎与单煎总皂苷在(5~400)μg·mL^(-1) 浓度范围内、总黄酮和总木脂素在(5~100)μg·mL^(-1) 浓度范围内、总生物碱在(5~800)μg·mL^(-1) 浓度范围内均对HCG-27细胞表现为低浓度促进,高浓度抑制,无细胞毒作用。结论根据细胞学药性评价法,厚朴温中汤合煎与单煎总提物及其各组分均表现为热性,厚朴温中汤合煎与单煎药性基本一致。
OBJECTIVE To investigate the consistency between the combined decoction of HouPu WenZhong Tang and the single decoction,and to explore the applicability of the formulated granules to replace the traditional soup.METHODS Dialysis and column chromatography were used to obtain the total extracts of HouPu WenZhong Tang and single decoction,as well as the total polysaccharides,total saponins,total flavonoids,total lignans and total alkaloids.The CCK-8 method was used to study the effects of the total extracts of the combined decoction and single decoction of HouPu WenZhong Tang and its components on the proliferation of human gastric cancer HCG-27 cells in vitro,and the effects of the drugs on the cell morphology were observed by microscopy,and the toxicity of the drugs on the cells was observed by Taipan blue staining.RESULTS The effects of the total extracts and total polysaccharides of the combined decoction and the single decoction on the proliferation of HCG-27 cells in the concentration range of 5-800μg·mL^(-1) were all promotional without cytotoxic effects.The total saponins of the combined decoction and single decoction in the concentration range of 5-400μg·mL^(-1),the total flavonoids and total lignans in the concentration range of 5-100μg·mL^(-1) ,and the total alkaloids in the concentration range of 5-800μg·mL^(-1) showed promotion at low concentrations and inhibition at high concentrations without cytotoxicity on HCG-27 cells.CONCLUSION According to the cytological drug property evaluation method,the total extract and its components of the combined decoction and single decoction of HouPu WenZhong Tang showed thermal properties,and the drug properties of the combined decoction and single decoction of HouPu WenZhong Tang were basically the same.
作者
顾子娴
陈家毅
石宣宜
欧阳莉
章芝欣
贾九青
刘建群
WenZhong Tang GU Zi-xian;CHEN Jia-yi;SHI Xuan-yi;OU Yang-li;ZHANG Zhi-xin;JIA Jiu-Qing;LIU Jian-qun(Key Laboratory of Modern Preparation of Traditional Chinese Medicine(TCM),Ministry of Education,Jiangxi University of TCM,Nanchang 330004,China)
出处
《海峡药学》
2024年第7期23-29,共7页
Strait Pharmaceutical Journal
基金
国家自然科学基金项目(82160731)
江西中医药大学校级科技创新团队发展计划(CXTD22007)。
关键词
厚朴温中汤
合煎
单煎
药性
一致性
HouPu WenZhong Tang
Combined decoction
Single decoction
Medicinal properties
Consistency
