摘要
马铃薯是干旱敏感作物,干旱严重影响其产量和品质。我们前期工作发现马铃薯类胡萝卜素裂解双加氧酶(carotenoid cleavage dioxygenase,CCD)基因(StCCD)高响应干旱胁迫。本研究基于马铃薯在干旱-复水-再干旱过程中的转录组和miRNA组测序结果,在全基因组层面上对马铃薯CCD基因家族及其互作的miRNAs进行了鉴定、生物信息学及表达谱分析,并检测了干旱-复水循环过程中干旱响应的StCCDs及其互作miRNAs的共表达变化。结果表明,在马铃薯中鉴定到17个CCD基因,定位于5条染色体上,可分为6个亚家族,编码174~1115个氨基酸残基的多肽,均具有保守基序motif 5。表达谱分析结果表明,StCCD家族17个基因在马铃薯植株不同组织和器官、多种胁迫及不同植物生长调节剂处理下均有表达,并呈现显著的差异化表达趋势,提示StCCD家族各基因功能的多样化作用。其中StCCD家族中StNCED1、StNCED6和StCCD4节律性响应干旱-复水循环,暗示着这3个成员是StCCD基因家族中调控干旱胁迫响应与适应的关键基因。结合miRNAs组测序数据和生物信息学预测,发现有777个miRNAs靶向调控StCCD基因家族的17个成员。共表达分析结果表明,对干旱高响应的StCCDs基因和相应miRNAs表达总体呈负调控关系,且这种互作可能参与了马铃薯对干旱胁迫的响应与适应过程。研究结果有助于解析StCCD家族的多重功能,以及StCCDs和相应的miRNAs的互作如何参与马铃薯对干旱胁迫的响应和适应机制,也可为后续马铃薯抗旱精准分子育种提供候选基因。
Potato is a kind of drought-sensitive crop,and drought severely affects its yield and quality.Our previous work found that the potato carotenoid cleavage dioxygenase(CCD)gene(StCCD)was highly responsive to drought stress.In this study,based on the sequencing results of potato transcriptome and miRNAome during the drought-rehydration-redrought process,the identification,bioinformatics analysis,and expression profiling of the potato CCD gene family and its interacting miRNAs were conducted at the whole-genome level.Co-expression analysis was also performed to investigate the interaction between drought-responsive StCCDs and their interacting miRNAs during the drought-rehydration cycles.The results showed that 17 CCD genes were identified in potato,located on five chromosomes and divided into six subfamilies.These genes encoded polypeptides ranging from 174 to 1115 amino acid residues and shared conserved motif 5.Expression profiling analysis revealed that the 17 StCCDs were expressed in different tissues and organs of potato plants,as well as under various stresses and plant growth regulator treatments,which demonstrated significant differential expression patterns,indicating the functional diversification of gene members of StCCD family.Among them,StNCED1,StNCED6,and StCCD4 in the StCCD family exhibited rhythmic responses to drought-rehydration cycles,suggesting that the three members are key genes regulating response and adaptation of drought stress in the StCCD family.Combining miRNAome sequencing data and bioinformatics prediction,777 miRNAs were found to target 17 members of the StCCD gene family.Co-expression analysis showed an overall negative regulatory relationship between the drought-responsive StCCDs and their corresponding miRNAs,indicating that the interaction between these miRNAs and their targeted StCCDs may be involved in the response and adaptation of potato to drought stress.The results of this study contribute to the understanding of the multifunctionality of the St-CCD gene family and the involvement of StCCDs and their corresponding miRNAs in the response and adaptation mechanisms of potato to drought stress,and provide candidate genes for subsequent precise molecular breeding of drought-tolerant potatoes.
作者
张艳红
张作胜
李洁沁
易靖
么梦凡
兰珊珊
龚明
ZHANG Yanhong;ZHANG Zuosheng;LI Jieqin;YI Jing;YAO Mengfan;LAN Shanshan;GONG Ming(School of Life Sciences,Yunnan Normal University,Southwest United Graduate School,Key Laboratory of Potato Biology of Yunnan Province,Engineering Research Center of Sustainable Development and Utilization of Biomass Energy,Ministry of Education,Key Laboratory of Biomass Energy and Environmental Biotechnology of Yunnan Province,Kunming 650500,China;Institute of Quality Standards and Testing Technology,Yunnan Academy of Agricultural Sciences,Kunming 650031,China)
出处
《植物生理学报》
CAS
CSCD
北大核心
2024年第6期937-954,共18页
Plant Physiology Journal
基金
国家自然科学基金(32260459)
云南省马铃薯生物学重点实验室开放基金(YNPKF202202和YNPKF202203)。
