摘要
桃蛀果蛾是严重隐蔽危害的蛀果害虫,亟待开发环境友好型防治方法,昆虫行为调控技术被认为是新型绿色的害虫防控手段,明晰嗅觉识别机制是开发行为调控剂的基础。基于桃蛀果蛾触角转录组得到242个嗅觉基因,基于序列比对和近缘物种相似基因的功能背景,选取了13个嗅觉基因,采用RT-qPCR进一步分析其潜在识别功能。结果表明,13个嗅觉基因中有6个基因在雌雄虫不同发育时期的表达水平存在显著差异(P<0.05),推测这6个基因与成虫期嗅觉识别行为相关;根据雌雄成虫初羽化、成熟未交尾和交尾后基因表达水平的差异推测,OBP11可能在识别性信息素和交配行为中发挥重要作用,OR45可能与雌虫识别寄主植物和产卵过程相关,IR5可能与交配和产卵行为相关。以上研究结果为解析桃蛀果蛾嗅觉识别机制奠定了基础,同时也为开发新型昆虫行为调控剂、构建环境友好的害虫治理策略提供了思路。
Carposina sasakii Matsumura is a notable fruit pest that damages many types of fruit trees.Behavior regulation technology has gradually gained attraction as a potential pest control method that is both environmentally conscious and effective.The insect behavioral mechanism of chemosensory perception is the basis of behavior regulation.A total of 242 olfactory genes were identified in the antenna transcriptome of the C.sasakii.Based on sequence alignment and functional background of similar genes in related species,13 olfactory genes were selected and their potential recognition functions were analyzed using RT-qPCR.The results showed that 6 genes differentially expressioned between males and females at different developmental stages(P<0.05),which corresponded to the behavioral phenotypes of adults.According to the difference expression levels between male and female adults during newly emerged,mature unmated and mature mated,OBP11 might play a role in detecting pheromones and mating behavior,OR45 might correspond to host-seeking and oviposition activities and IR5 was associated with mating and oviposition behavior.Above results laid theoretical molecular foundation for the identification of C.sasakii pheromone and olfactory-related genes and supported the implementation of behavioral regulators against the olfactory perception mechanism of C.sasakii as a more environmentally sustainable pest control strategy.
作者
张倩
门丽娜
李一然
刘巧
Angie DENG
胡晓雯
张宇宏
张志伟
张伟
ZHANG Qian;MEN Lina;LI Yiran;LIU Qiao;Angie DENG;HU Xiaowen;ZHANG Yuhong;ZHANG Zhiwei;ZHANG Wei(College of Forestry,Shanxi Agricultural University,Shanxi Taigu 030801,China;Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China;Lucile Packard Children’s Hospital at Stanford,Palo Alto,California 94303,USA)
出处
《中国农业科技导报》
CAS
CSCD
北大核心
2024年第8期151-162,共12页
Journal of Agricultural Science and Technology
基金
National Natural Science Foundation of China(32171806)
Research Project Supported by the Shanxi Scholarship Council of China(2021-067)
Shanxi Basic Research Project(20210302123392,20210302124155)
Shanxi Forest and Grass Key R&D Program Special Project
Graduate Student Innovation Project of Shanxi Province(2021Y327).
