摘要
为研究B细胞淋巴瘤2(B-cell lymphoma-2,Bcl-2)对宰后秦川牛肉细胞凋亡及保水性的调控作用。用200μmol/L甘氨鹅脱氧胆酸(glycochenodeoxycholic acid,GCDA)和10μmol/L 2-乙基-3-甲基戊酰胺(2-ethyl-3-methylpentanamide,ABT-737)溶液注射处理秦川牛背最长肌,测定并分析在4℃条件下分别成熟0、6、12、24、48、72、120 h时,Bcl-2、Bcl-2关联X蛋白含量、内质网和线粒体钙离子浓度、电压依赖性阴离子通道蛋白1(voltage-dependent anion channel protein 1,VDAC1)表达量、钙蛋白酶含量以及线粒体细胞凋亡途径和保水性等相关指标变化情况。结果表明,在宰后6 h,GCDA处理组的Bcl-2含量极显著大于对照组(P<0.01),并且其内质网钙离子浓度均极显著低于对照组(P<0.01),两个处理组的蒸煮损失率均显著低于对照组(P<0.05)。在宰后12 h和24 h,ABT-737处理组的Bcl-2含量极显著低于对照组(P<0.01)。在宰后12 h和120 h,ABT-737处理组的内质网、线粒体钙离子浓度和钙蛋白酶均极显著高于对照组(P<0.01)。宰后0~120 h,两组处理组的VDAC1总表达量低于对照组。综上,秦川牛宰后成熟过程中,在Bcl-2的调控下,通过VDAC1通道运输发生钙离子超载,造成钙蛋白酶的活化,线粒体内Ca^(2+)超载会促使半胱天冬酶(Caspase)-9和Caspase-3被激活,从而诱导细胞凋亡,细胞凋亡和Caspase激活程度越高,肌原纤维蛋白降解越多,持水能力越差,肉品保水性越低。
In order to investigate the regulatory effect of B-cell lymphoma-2 (Bcl-2) on cell apoptosis and water retention in postmortem Qinchuan beef,the longissimus dorsi muscle of Qinchuan cattle was injected with either 200μmol/L glycochenodeoxycholic acid (GCDA) or 10μmol/L 2-ethyl-3-methylpentanamide (ABT-737) solution and aged at 4℃postmortem.After 0,6,12,24,48,72 and 120 h,the changes in Bcl-2 and Bcl-2-associated X protein (Bax) contents,the concentrations of endoplasmic reticulum and mitochondrial calcium ion,voltage-dependent anion channel protein 1 (VDAC1)expression,calpain content,the mitochondrial apoptosis pathway and water retention were determined.The results showed that at 6 h after slaughter,the Bcl-2 content of the GCDA-treated group was significantly higher than that of the control group (P<0.01),the calcium ion concentration in the endoplasmic reticulum was significantly lower than that of the control group (P<0.01),and cooking loss was significantly lower in both treatment groups than the control group (P<0.05).At12 and 24 h post-slaughter,the Bcl-2 content of the ABT-737-treated group was significantly lower than that of the control group (P<0.01).At 12 and 120 h post-slaughter,the endoplasmic reticulum calcium ion,mitochondrial calcium ion and calpain levels in the ABT-737-treated group were significantly greater than those in the control group (P<0.01).During0–120 h post-slaughter,the total expression of VDAC1 in both treatment groups was lower than that in the control group.In summary,during the postmortem aging of Qinchuan beef,under the regulation of Bcl-2,calcium overload occurs through VDAC1-facilitated calcium transport,resulting in the activation of calpain,and the overloading of Ca~(2+)in mitochondria promotes the activation of caspase-9 and caspase-3,thus inducing cell apoptosis.The higher the degrees of cell apoptosis and caspase activation,the higher the degree of degradation of myofibrillar proteins,and the poorer the water-holding capacity of the meat.
作者
刘长玲
高爽
马佳荣
李亚蕾
罗瑞明
曹松敏
LIU Changling;GAO Shuang;MA Jiarong;LI Yalei;LUO Ruiming;CAO Songmin(School of Food Science and Engineering,Ningxia University,Yinchuan 750021,China;School of Animal Science and Technology,Ningxia University,Yinchuan 750021,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2024年第15期205-213,共9页
Food Science
基金
国家自然科学基金地区科学基金项目(32160535)。
