摘要
目的:分析石菖蒲炮制前后化学成分的差异,为石菖蒲及不同炮制品的质量控制提供依据。方法:采用气相色谱-质谱联用(GC-MS)法对石菖蒲及炮制品挥发性成分进行分析鉴定,根据峰面积归一化法计算各成分的相对含量。采用GC建立15批石菖蒲及炮制品的指纹图谱。利用相似度评价、聚类分析(HCA)、主成分分析(PCA)和正交偏最小二乘判别分析(OPLS-DA)等方法,评价石菖蒲炮制前后的质量差异。结果:GC-MS分析结果可知,石菖蒲及炮制品共鉴定出40个化合物,石菖蒲经酒制、姜制后,草蒿脑、甲基丁香酚,反式甲基丁香酚相对含量降低,榄香素、α-细辛醚和β-细辛醚相对含量升高。石菖蒲、酒石菖蒲、姜石菖蒲的指纹图谱分别标定了25、20、22个共有峰,石菖蒲及不同炮制品指纹图谱标定了28个共有峰。HCA可将石菖蒲及炮制品聚为3类,PCA结果提取了6个主成分,OPLS-DA能有效地将石菖蒲生品和不同炮制品明显区分,并筛选出了12种主要差异成分。结论:该研究建立的指纹图谱结合化学模式识别研究和GC-MS分析有助于石菖蒲及炮制品的区分及整体质量评价。
Objective:To analyze the difference of chemical composition of Acorus tatarinowii before and after processing,and to provide the basis for the quality control of Acorus tatarinowii and different processed products.Methods:The volatile components of Acorus tatarinosus and processed products were analyzed and identified by gas chromatography-mass spectrometry(GC-MS),and the relative content of each component was calculated according to the peak area normalization method.Fingerprints of 15 batches of Acorus tatarinosus and its processed products were established by GC.The similarity evaluation,cluster analysis(HCA),principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA)were used to evaluate the quality difference of Acorus tatarinosus before and after processing.Results:GC-MS analysis showed that a total of 40 compounds were identified in Acorus tatarinous and processed products.After acorus tatarinous was treated with wine and ginger,the relative contents of artemisinin,methyl eugenol and trans-methyl eugenol decreased,while the relative contents of elemin,α-asarone andβ-asarone increased.There were 25,20 and 22 common peaks in the fingerprint of Acorus tatarinosus,wine Acorus tatarinosus and Ginger Acorus tatarinosus,respectively.There were 28 common peaks in the fingerprint of Acorus tatarinosus and different processed products.HCA could classify the raw Acorus tatarinosus and its processed products into three categories,PCA results extracted six principal components,OPLS-DA could effectively distinguish the raw Acorus tatarinosus and different processed products,and 12 main differential components were screened out.Conclusion:The fingerprint established in this study combined with chemical pattern recognition study and GC-MS analysis is helpful for the discrimination and overall quality evaluation of Acorus tatarinosus and its processed products.
作者
杨双玲
薛艳芳
陈华师
金浩鑫
邹兆祚
李雪艳
饶毅
YANG Shuangling;XUE Yanfang;CHEN Huashi;JIN Haoxin;ZOU Zhaozuo;LI Xueyan;RAO Yi(Jiangxi University of Chinese Medicine,Nanchang 330004,China;Jiangxi Jianchangbang Pharmaceutical Co.,Ltd.,Nanchang 330038,China;National Engineering Research Center for Manufacturing Technology of Traditional Chinese Medicine Solid Preparations,Nanchang 330006,China)
出处
《江西中医药大学学报》
2024年第4期65-71,76,共8页
Journal of Jiangxi University of Chinese Medicine
基金
江西省中医药管理局科技项目(2022B1023)
江西中医药大学校级科技创新团队发展计划项目(CXTD22015)。
关键词
石菖蒲
挥发油
GC-MS
指纹图谱
化学识别模式
质量评价
Tatarinowii
Volatile Oil
GC-MS
Fingerprint
Chemical Recognition Mode
Assessment of Quality
