摘要
为分析人工诱导星康吉鳗卵巢发育组织脂质代谢,实验使用超高效液相色谱四极杆轨道阱质谱(UHPLC/Q-Orbitrap MS)对人工诱导星康吉鳗卵巢发育不同时期肝脏、卵巢、肌肉和血浆进行了脂质组学分析,并测定了肝脏代谢酶活。对不同发育阶段星康吉鳗组织脂质成分分析结果显示,在肝脏、肌肉、卵巢和血浆中各鉴定出68、27、63和212种脂质标志物,主要包括磷脂酰丝氨酸(PS)、磷脂酰乙醇胺(PE)、磷脂酰胆碱(PC)、甘油三酯(TG)和鞘磷脂(SM)等。4种组织中TG、甘油二酯(DG)和神经酰胺(Cer)等脂类物质在发育过程中呈现出不同的变化趋势。肝脏代谢中,脂肪酸合成酶、肝脂酶和脂蛋白脂酶浓度呈现先升高后降低的过程,主要参与了TG、磷脂和脂肪酸的代谢。研究表明,星康吉鳗卵巢发育过程中主要组织的TG、磷脂发生了不同程度的变化,脂质代谢相关酶活性变化也与脂质代谢特征相对应。
Conger eel(Conger myriaster)is one of the common fish in the East China Sea and other coastal waters,with over fishing and pollution in the marine environment,the wild resources of C.myriaster face the risk of depletion.However,there are limit studies on the reproduction and a lack of research on lipid requirements during the development of the ovary of the C.myriaster.Therefore,lipid metabolism of artificially induced ovary development tissue of lipid metabolism was analyzed in different developmental stages of ovary in this study to provide a theoretical basis for artificial reproduction of the C.myriaster.This study provides insights into the lipid metabolism of the C.myriaster during artificially induced ovarian development.The lipid compositions of the liver,ovary,muscle and plasma in distinct stages were determined using ultra-high performance liquid chromatography coupled with quadrupole-orbitrap mass spectrometry(UHPLC/Q-Orbitrap MS).The activity of metabolic enzymes in the liver was investigated as well.By analyzing the lipid components of liver,ovary,muscle,and plasma of C.myriaster at different developmental stages,68,27,63 and 212 lipid markers were identified in the liver,muscle,ovary and plasma,mainly including phosphatidylserine(PS),phosphatidylethanolamine(PE),phosphatidylcholine(PC),triglycerides(TG)and sphingomyelin(SM).Lipids such as TG,diglyceride(DG)and ceramide(Cer)in the four tissues showed different trends during developments.In liver metabolism,the concentration of fatty acid syntheses,liver lipase and lipoprotein lipase showed a process of first increasing and then decreasing.The process was mainly involved in the metabolism of triglycerides,phospholipids and fatty acids.Studies showed that the triglycerides and phospholipids in the main tissues of the C.myriaster had changed to varying degrees during the development of the ovary,and the changes in the activities of lipid metabolism-related enzymes also corresponded to the characteristics of lipid metabolism.This experiment initially explored the lipid mobilization of main tissues during the ovary development of the C.myriaster to provide a basic reference for the artificial reproduction and sustainable development of the C.myriaster.
作者
王桂香
李慷
刘如聪
姜之信
巴旭冰
宋宗诚
刘利平
WANG Guixiang;LI Kang;LIU Rucong;JIANG Zhixin;BA Xubing;SONG Zongcheng;LIU Liping(Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Ministry of Education,Shanghai Ocean University,Shanghai 201306,China;Shanghai Engineering Research Center of Aquaculture,Shanghai Ocean University,Shanghai 201306,China;National Demonstration Center for Experimental Fisheries Science Education,Shanghai Ocean University,Shanghai 201306,China;Weihai Shenghang Aquatic Technology Limited Company,Weihai 264319,China)
出处
《水产学报》
CAS
CSCD
北大核心
2024年第8期89-99,共11页
Journal of Fisheries of China
基金
上海市科技兴农技术创新项目(2020-02-08-00-10-F01471)
国家自然科学基金(32072994)
上海海洋大学科技发展专项。
关键词
星康吉鳗
脂质转化
脂质组学
脂质代谢
Conger myriaster
lipid conversion
lipidomics
liver metabolism
